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    products/proteins-peptides/recombinant-human-sqstm1--p62-protein-ab132366.pdf

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Recombinant Human SQSTM1 / p62 protein (ab132366)

  • Datasheet
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SDS-PAGE - Recombinant Human SQSTM1 / p62 protein (ab132366)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: SDS-PAGE, WB, ELISA

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    Description

    • Product name

      Recombinant Human SQSTM1 / p62 protein
      See all SQSTM1 / p62 proteins and peptides
    • Expression system

      Wheat germ
    • Accession

      Q13501
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSR VAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIK EKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDL CSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEM GPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSP LGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNV EGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPST GELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQ MLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
      • Predicted molecular weight

        74 kDa including tags
      • Amino acids

        1 to 440

    Associated products

    • Related Products

      • Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab109012)
      • Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      • Anti-SQSTM1 / p62 antibody (ab91526)

    Specifications

    Our Abpromise guarantee covers the use of ab132366 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

      ELISA

    • Form

      Liquid
    • Additional notes

       

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • A170
      • DMRV
      • EBI 3 associated protein of 60 kDa
      • EBI 3 associated protein p60
      • EBI3 associated protein of 60 kDa
      • EBI3 associated protein p60
      • EBI3-associated protein of 60 kDa
      • EBIAP
      • FTDALS3
      • MGC127197
      • ORCA
      • OSF-6
      • Osi
      • OSIL
      • Oxidative stress induced like
      • p60
      • p62
      • p62B
      • Paget disease of bone 3
      • PDB 3
      • PDB3
      • Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa
      • Phosphotyrosine independent ligand for the Lck SH2 domain p62
      • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
      • PKC-zeta-interacting protein
      • Protein kinase C-zeta-interacting protein
      • Sequestosome 1
      • Sequestosome-1
      • SQSTM 1
      • SQSTM_HUMAN
      • Sqstm1
      • STAP
      • STONE14
      • Ubiquitin binding protein p62
      • Ubiquitin-binding protein p62
      • ZIP
      • ZIP 3
      • ZIP3
      see all
    • Function

      Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
    • Tissue specificity

      Ubiquitously expressed.
    • Involvement in disease

      Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.
    • Sequence similarities

      Contains 1 OPR domain.
      Contains 1 UBA domain.
      Contains 1 ZZ-type zinc finger.
    • Domain

      The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.
      The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.
      The ZZ-type zinc finger mediates the interaction with RIPK1.
    • Post-translational
      modifications

      Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.
    • Cellular localization

      Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.
    • Target information above from: UniProt accession Q13501 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human SQSTM1 / p62 protein (ab132366)
      SDS-PAGE - Recombinant Human SQSTM1 / p62 protein (ab132366)
      12.5% SDS-PAGE analysis of ab132366 stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab132366? Please let us know so that we can cite the reference in this datasheet.

    ab132366 has been referenced in 1 publication.

    • Aki T  et al. Formation of high molecular weight p62 by CORM-3. PLoS One 14:e0210474 (2019). PubMed: 30620762

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