Recombinant Human STUB1/CHIP protein (ab82791)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human STUB1/CHIP protein
See all STUB1/CHIP proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MKGKEEKEGG ARLGAGGGSP EKSPSAQELK EQGNRLFVGR KYPEAAACYG RAITRNPLVA VYYTNRALCY LKMQQHEQAL ADCRRALELD GQSVKAHFFL GQCQLEMESY DEAIANLQRA YSLAKEQRLN FGDDIPSALR IAKKKRWNSI EERRIHQESE LHSYLSRLIA AERERELEEC QRNHEGDEDD SHVRAQQACI EAKHDKYMAD MDELFSQVDE KRKKRDIPDY LCGKISFELM REPCITPSGI TYDRKDIEEH LQRVGHFDPV TRSPLTQEQL IPNLAMKEVI DAFISENGWV EDY -
Amino acids
1 to 303
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab82791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
Previously labelled as STUB1.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.50
Constituents: 0.077% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Antigen NY CO 7
- Antigen NY-CO-7
- C terminus of Hsp70-interacting protein
see all -
Function
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. -
Tissue specificity
Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 3 TPR repeats.
Contains 1 U-box domain. -
Domain
The TPR domain is essential for ubiquitination mediated by UBE2D1. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human STUB1/CHIP protein (ab82791)15% SDS-PAGE showing ab82791 at approximately 34.8kDa (3µg).
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Western blot - Recombinant Human STUB1/CHIP protein (ab82791)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab82791 has been referenced in 1 publication.
- Labrador-Garrido A et al. Chaperome screening leads to identification of Grp94/Gp96 and FKBP4/52 as modulators of the a-synuclein-elicited immune response. FASEB J 30:564-77 (2016). PubMed: 26443817