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    products/proteins-peptides/recombinant-human-tgf-beta-1-protein-active-ab50036.pdf

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Bioactive grade

Recombinant human TGF beta 1 protein (Active) (ab50036)

  • Datasheet
  • SDS
Submit a review Q&A (19)References (39)

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Western blot - Recombinant human TGF beta 1 protein (Full length) (ab50036)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 98% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: HPLC, SDS-PAGE, Functional Studies, WB

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    Description

    • Product name

      Recombinant human TGF beta 1 protein (Active)
      See all TGF beta 1 proteins and peptides
    • Biological activity

      The ED50, as determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells, is ≤ 0.05 ng/ml), corresponding to a specific activity of ≥ 2 x 107 units/mg.

    • Purity

      > 98 % SDS-PAGE.
      > 98% by HPLC analyses. Endotoxin Level: < 0.1 ng/µg of protein (< 1 EU/µg).
    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      HEK 293 cells
    • Accession

      P01137
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
      • Predicted molecular weight

        25 kDa
      • Amino acids

        279 to 390
      • Additional sequence information

        TGF-beta1 is a 25.0 kDa protein with each subunit containing 112 aa, linked by a single disulfide bond. It is the mature protein after cleavage of the signal peptide and latency-associated peptide.

    Specifications

    Our Abpromise guarantee covers the use of ab50036 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      HPLC

      SDS-PAGE

      Functional Studies

      Western blot

    • Form

      Lyophilized
    • Additional notes

      TGF-beta is secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix.

      The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1.

      TGF-β1 is the most abundant isoform secreted by almost every cell type.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Centrifuge the vial prior to opening. Reconstitute in 10mM Citric Acid, pH3.0 to a concentration of 50 µg/ml (e.g. 5 µg/100µl). This solution can then be stored at room temperature or at 4oC for 1 month. It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at -20oC for future use.

    General Info

    • Alternative names

      • Cartilage-inducing factor
      • CED
      • Differentiation inhibiting factor
      • DPD1
      • LAP
      • Latency-associated peptide
      • Prepro transforming growth factor beta 1
      • TGF beta
      • TGF beta 1
      • TGF beta 1 protein
      • TGF-beta 1 protein
      • TGF-beta-1
      • TGF-beta-5
      • TGF-beta1
      • TGFB
      • Tgfb-1
      • tgfb1
      • TGFB1_HUMAN
      • TGFbeta
      • TGFbeta1
      • Transforming Growth Factor b1
      • Transforming Growth Factor beta 1
      • Transforming growth factor beta 1a
      • transforming growth factor beta-1
      • transforming growth factor, beta 1
      • Transforming Growth Factor-ß1
      see all
    • Function

      Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
    • Tissue specificity

      Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
    • Involvement in disease

      Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
    • Sequence similarities

      Belongs to the TGF-beta family.
    • Post-translational
      modifications

      Glycosylated.
      The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P01137 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Recombinant human TGF beta 1 protein (Full length) (ab50036)
      Western blot - Recombinant human TGF beta 1 protein (Full length) (ab50036)
      All lanes : Anti-CTGF antibody [EPR20728] (ab209780) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast) starved for 18 hours, whole cell lysate
      Lane 2 : NIH/3T3 starved for 18 hours, then treated with 10 ng/ml transforming growth factor-ß (TGF-ß1, ab50036) and 50 µg/ml Heparin sodium salt for 24 hours, whole cell lysate
      Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution


      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

      The level of CTGF expression can be induced by TGF beta treatment (PMID: 17786299).

      CTGF is constitutively expressed in HepG2 cells (PMID:15886528).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (39)

    Publishing research using ab50036? Please let us know so that we can cite the reference in this datasheet.

    ab50036 has been referenced in 39 publications.

    • Cai H  et al. Overexpressed integrin alpha 2 inhibits the activation of the transforming growth factor ß pathway in pancreatic cancer via the TFCP2-SMAD2 axis. J Exp Clin Cancer Res 41:73 (2022). PubMed: 35193647
    • Zhong R  et al. Regulation of Ion Channel Function in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes by Cancer Cell Secretion Through DNA Methylation. Front Cardiovasc Med 9:839104 (2022). PubMed: 35265687
    • Farooqi HMU  et al. Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system. Nano Converg 8:3 (2021). PubMed: 33528697
    • Turan E  et al. L-carnitine suppresses transient receptor potential vanilloid type 1 activity and myofibroblast transdifferentiation in human corneal keratocytes. Lab Invest 101:680-689 (2021). PubMed: 33637945
    • Hernandez I  et al. Human Placental NADPH Oxidase Mediates sFlt-1 and PlGF Secretion in Early Pregnancy: Exploration of the TGF-ß1/p38 MAPK Pathways. Antioxidants (Basel) 10:N/A (2021). PubMed: 33673360
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 19 Abreviews or Q&A

    Question

    Inquiry: Hello, I am looking for a preparation of TGFB-1 to use for in vitro induction of EMT in human cell lines. Which of your products would you recommend to this kind of assay? Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 31 2012

    Answer

    Thank you for your enquiry.

    I have reviewed with our sources the TGF beta 1 proteins that we have on our catalog. It seems the most suitable would be ab50036 TGF beta 1 protein (Active).

    https://www.abcam.com/Recombinant-human-TGF-beta-1-protein-Active-ab50036.html (or use the following: https://www.abcam.com/Recombinant-human-TGF-beta-1-protein-Active-ab50036.html).

    This should be suitable for use on human cells, as it is a human protein. Unfortunately, I am sorry we do not have any information if it has been used specifically for in the in vitro induction of EMT in human cell lines, but there are many references on our website for this product which state usage of this product in a variety of applications.

    https://www.abcam.com/TGF-beta-1-protein-Active-ab50036-references.html

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Oct 31 2012

    Question

    I do not have Citric acid available in my lab. Is there an alternative acid that I can reconstitute this in (such as HCl)?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 22 2014

    Answer

    HCl can be used as an alternative at the same recommended molarity and pH (10 mM, pH 3).

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on Dec 22 2014

    Question

    I see you recommend reconstitution in Citric acid. In order to conduct my experiment and make comparisons across cytokines that I am reconstituting in acetic acid, I was hoping to be able to reconstitute ab50036 in acetic acid instead. Do you foresee any issues with this or have any recommendations?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 01 2014

    Answer

    It is important to strictly follow the reconstitution protocol on the datasheet. Reconstituting our TGF-β1 in acetic acid instead of the recommended citric acid will not fully dissolve this product in solution which may adversely affect its performance in an assay.

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on Aug 01 2014

    Question



    Inquiry: How to do reconstitution of ab50036 and ab84070. Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on May 07 2013

    Answer

    Thank you for contacting us. Reconstitution instructions for the proteins are below.

    Centrifuge the vial prior to opening. Reconstitute in 10mM Citric Acid, pH 3.0 to a
    concentration of 0.1-1.0 mg/ml. Do not vortex. For
    extended storage, it is recommended to further dilute in a
    buffer containing a carrier protein (example 0.1% BSA) and
    store in working aliquots at -20oC to -80oC.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on May 07 2013

    Question

    I want to use VEGF protein (ab51967) to inoculate it in lab. animals (rats) . I want to reach to a dilution 1 µg/ 100 µl and 1 µg/ 1 µl. How can I do that? which diluent can I use? the same thing for TGF beta 1 protein (Active) (ab50036).

    Read More

    Abcam community

    Verified customer

    Asked on Feb 18 2013

    Answer


    Reconstitution of ab50036 should be performed as indicated on the datasheet:
    1. Centrifuge the vial prior to opening.
    2. Reconstitute in 10mM Citric Acid, pH 3.0 to a concentration of 0.1-1.0 mg/ml. Do not vortex.
    3. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.
    These conditions are optimized to ensure complete solubility and required bioactivity of Human TGF-β1. I would advise to strictly follow the protocol to avoid loss of activity.

    Below is an article published in 2009 where the authors used a Human TGF-β1 in vivo. Reviewing this article may be helpful for further information.
    Freeman, I The influence of the sequential delivery of angiogenic factors from affinity-binding alginate scaffolds on vascularization. Biomaterials;30(11):2122-31. 17 Jan 2009. PMID 19152972.

    Read More

    Abcam Scientific Support

    Answered on Feb 18 2013

    Question

    I want to use VEGF protein (ab51967) to inoculate it in lab. animals (rats) . I want to reach to a dilution 1 µg/ 100 µl and 1 µg/ 1 µl. How can I do that? which diluent can I use? the same thing for TGF beta 1 protein (Active) (ab50036).

    Read More

    Abcam community

    Verified customer

    Asked on Jan 15 2013

    Answer


    ab51967 is delivered in 10ug. First, make the 1ug/ul solution by diluting the 10ug in 10ul of water. Then, take 1ul of that solution and dilute it in 99ul of water to make the 0.01 ug/ul solution.
    ab50036 is delivered in 5ug. First, make the 1ug/ul solution by diluting the 5ug in 5ul of 10mM Citric Acid, ph3.0. Then, take 1ul of that solution and dilute it in 99ul of 10mM Citric Acid, ph3.0 to make the 0.01 ug/ul solution. For longer term storage at -20oC of the 0.01 ug/ul solution, 99ul of PBS containing 2 mg/ml albumin can be used instead of 99ul of 10mM Citric Acid.

    Read More

    Abcam Scientific Support

    Answered on Jan 15 2013

    Question

    Dear All,



    Are those proteins below injectable in Mice?




    1. SPARC protein (MBP) (ab92965)



    2. TGF beta 1 protein (Active) (ab50036)




    3. VEGF protein (Active) (ab9571)

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2012

    Answer

    Thank you for contacting us and for your patience.

    I had to contact the laboratory in order to get more information about the use of these three proteins.

    I can confirm, that for the ab92965 SPARC protein (MBP) to our knowledge it has not been tested for injection in mice or other animals (besides it uses as immunogen for polyclonal antibodies production in rabbits intramuscular). It could be that it will work for injection in mice but we can not guarantee this and we have no information about different administration routes (intravenous, intramuscular etc.).

    The two other proteins have been used in mice before. See the following links:

    1. ab9571 VEGF protein (Active)

    http://www.ncbi.nlm.nih.gov/pubmed/19088027

    http://www.ncbi.nlm.nih.gov/pubmed/10784613

    http://www.ncbi.nlm.nih.gov/pubmed/11689883

    http://www.ncbi.nlm.nih.gov/pubmed/12036930

    http://www.ncbi.nlm.nih.gov/pubmed/9401980

    http://www.ncbi.nlm.nih.gov/pubmed/15358628

    http://www.ncbi.nlm.nih.gov/pubmed/16477043

    http://www.ncbi.nlm.nih.gov/pubmed/16888632

    http://www.pnas.org/content/104/8/2837.full

    http://www.ncbi.nlm.nih.gov/pubmed/18180379

    http://www.ncbi.nlm.nih.gov/pubmed/15067321

    http://www.ncbi.nlm.nih.gov/pubmed/20056792

    http://www.ncbi.nlm.nih.gov/pubmed/22025303

    http://www.pnas.org/content/early/2011/07/25/1016546108.abstract



    2. ab50036 TGF beta 1 protein (Active)

    http://www.ncbi.nlm.nih.gov/pubmed/20304823

    http://www.ncbi.nlm.nih.gov/pubmed/22761317

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Dec 20 2012

    Question

    The below customer has bought this item have some doubt .

    He is asking how he can be sure that he has received the 5ug products after reconstitution as he could not see anything.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 07 2012

    Answer

    Thank you for contacting us.


    I like to reassure that if there was no protein in the vial that will be covered by our guarantee and we will be pleased to provide a credit note, free of charge replacement or refund if that is this case.

    I can confirm that 5 ug is a very small amount. It can not necessarily be seen even after centrifugation.


    Could you kindly provide the result of the earlier discussed SDS-PAGE? The result should confirm rather there is some protein in the vial or not.

    I hope this information is helpful to you and looking forward to receive the requested information.


    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 07 2012

    Question

    if i filter sterilize this product will it affect the activity?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 05 2012

    Answer

    Thank you for contacting us. This TGF beta 1 protein (ab50036) has been sterile filtered through a 0.2 micron filter prior to lyophilization and it would therefore not be necessary to sterilize it again. Filtration with a 0.2 micron filter will not have any effect on the activity, however. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on Nov 05 2012

    Question

    Can you please tell me if cat#ab50036 has any carrier proteins added to the preparation and, also, what was in the buffer before lyophylization?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 02 2012

    Answer

    Thank you for contacting us.

    The TGF beta 1 protein ab50036 is supplied carrier-free and was lyophilized from a solution containing 0.1% trifluoroacetic acid.

    For reconstitution, the recommendation for this lot is:

    Centrifuge the vial prior to opening. Reconstitute in 10mM Citric Acid, pH 3.0 to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 02 2012

    1-10 of 19 Abreviews or Q&A

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