Recombinant human TGF beta 2 protein (Active) (ab277760)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: MS, SDS-PAGE, HPLC, Functional Studies, Cell Culture
Description
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Product name
Recombinant human TGF beta 2 protein (Active)
See all TGF beta 2 proteins and peptides -
Biological activity
Fully biologically active determoned by the dose-dependent inhibition of IL-4 dependent HT-2 cell proliferation.
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Purity
>= 95 % SDS-PAGE.
=95% by HPLC -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Carrier free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
LSTCSTLDMD QFMRKRIEAI RGQILSKLKL TSPPEDYPEP EEVPPEVISI YNSTRDLLQE KASRRAAACE RERSDEEYYA KEVYKIDMPP FFPSENAIPP TFYRPYFRIV RFDVSAMEKN ASNLVKAEFR VFRLQNPKAR VPEQRIELYQ ILKSKDLTSP TQRYIDSKVV KTRAEGEWLS FDVTDAVHEW LHHKDRNLGF KISLHCPCCT FVPSNNYIIP NKSEELEARF AGIDGTSTYT SGDQKTIKST RKKNSGKTPH LLLMLLPSYR LESQQTNRRK KRALDAAYCF RNVQDNCCLR PLYIDFKRDL GWKWIHEPKG YNANFCAGAC PYLWSSDTQH SRVLSLYNTI NPEASASPCC VSQDLEPLTI LYYIGKTPKI EQLSNMIVKS CKCS -
Predicted molecular weight
46 kDa -
Actual molecular weight
46 kDa -
Molecular weight information
Predicted mass is 45592.13 Da (+/- 1-Da by ESI TOF). Multiple peaks are due to proteolytic cleavage during expression. Peak1 (12720 Da, aa303-414); Peak 2 (32170 DA,aa 21-297; Peak 3 (45592 Da, aa 21-414). -
Amino acids
21 to 414 -
Additional sequence information
N-terminal glycine tag. Full-length chain lacking the signal peptide.
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Specifications
Our Abpromise guarantee covers the use of ab277760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
HPLC
Functional Studies
Cell Culture
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
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Alternative names
- BSC-1 cell growth inhibitor
- BSC1 cell growth inhibitor
- Cetermin
see all -
Function
TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. -
Involvement in disease
A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.
Loeys-Dietz syndrome 4
Defects in TGFB2 may be a cause of non-syndromic aortic disease (NSAD). NSAD is a frequently asymptomatic but potentially lethal disease characterized by thoracic aortic aneurysms and dissections without additional syndromic features. -
Sequence similarities
Belongs to the TGF-beta family. -
Post-translational
modificationsThe precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human TGF beta 2 protein (ab277760)
Fully biologically active determoned by the dose-dependent inhibition of IL-4 dependent HT-2 cell proliferation.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3373524-1
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SDS-PAGE - Recombinant Human TGF-beta 2 protein (active) (ab277760)SDS-PAGE analysis of ab277760.
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Mass Spectrometry - Recombinant Human TGF-beta 2 protein (active) (ab277760)Mass determination by ESI TOF.
Predicted mass is 45592.13 Da (+/- 1-Da by ESI TOF). Multiple peaks are due to proteolytic cleavage during expression. Peak1 (12720 Da, aa303-414); Peak 2 (32170 DA,aa 21-297; Peak 3 (45592 Da, aa 21-414).
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HPLC - Recombinant Human TGF-beta 2 protein (active) (ab277760)HPLC analysis of ab277760.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab277760 has been referenced in 1 publication.
- Shi G & Yang F Krüppel-like factor 1 (KLF1) promoted the proliferation, migration and invasion of human lens epithelial cells by enhancing the expression of Zinc Finger and BTB Domain Containing 7A (ZBTB7A) and activating Wnt/ß-catenin pathway. Bioengineered 12:4374-4384 (2021). Functional Studies . PubMed: 34304709
