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Recombinant human TGF beta 3 protein (Active) (ab52313)

Submit a review Q&A (2)References (1)
Functional Studies - Recombinant human TGF beta 3 protein (Active) (ab52313)
  • Western blot - Recombinant human TGF beta 3 protein (ab52313)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Active: Yes
  • Suitable for: HPLC, WB, Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human TGF beta 3 protein (Active)
    See all TGF beta 3 proteins and peptides

  • Biological activity

    ab52313 is fully biologically active.

    The ED50 was determined by cytotoxicity assay using the WHO standard 98/608 as a direct comparison is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 108 units/mg.

  • Purity

    > 98 % SDS-PAGE.
    >98% HPLC analyses

  • Expression system

    Escherichia coli

  • Accession

    P10600

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS

    • Predicted molecular weight

      26 kDa

    • Amino acids

      301 to 412

    • Additional sequence information

      (Gene symbol: TGFB3) (Gene ID: 7043)

Specifications

Our Abpromise guarantee covers the use of ab52313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    This product is manufactured by BioVision, an Abcam company and was previously called 4344 TGF-beta3, human recombinant. 4344-5 is the same size as the 5 µg size of ab52313.

    Endotoxin level: <0.1 ng/μg.

    Centrifuge the vial prior to opening.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Please see notes section.

    Constituents: 20% Ethanol, 0.06% Acetic acid buffer

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • ARVD
    • ARVD1
    • FLJ16571
    • LDS5
    • MGC105479
    • MGC118722
    • prepro-transforming growth factor beta-3
    • RNHF
    • TGF beta 3
    • TGF beta3
    • TGF-beta-3
    • TGFB 3
    • Tgfb3
    • TGFB3_HUMAN
    • transforming growth factor beta 3
    • Transforming growth factor beta-3
    see all

  • Function

    Involved in embryogenesis and cell differentiation.

  • Involvement in disease

    Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human TGF beta 3 protein (Active) (ab52313)
    Functional Studies - Recombinant human TGF beta 3 protein (Active) (ab52313)

    Cytotoxicity Assay of ab52313.

  • Western blot - Recombinant human TGF beta 3 protein (ab52313)
    Western blot - Recombinant human TGF beta 3 protein (ab52313)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab52313? Please let us know so that we can cite the reference in this datasheet.

ab52313 has been referenced in 1 publication.

  • Ye P Modulation of epithelial tight junctions by TGF-beta 3 in cultured oral epithelial cells. Aust Dent J 57:11-7 (2012). PubMed: 22369552

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

We received a TGFbeta 3 (ab52313). It is written on your shipment paper : stored at 4C, stable for at least 3 months. After reconstitution, store at -20C.
When we received this TGF, it has been directly stored at -20C (without any reconstitution). Do you think it can have an impact on the stability/concentration of the protein?

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Answer

The composition of the storage buffer of the ab52313 is 20% Ethanol and 0.1% Acetate.
Since there is 20% ethanol in the solution, hopefully the protein might not be fully frozen at -20C. Therefore, there is a possibility that the protein still works. However, there might be some decrease in the biological activity but overall it might be worthwhile using it. We would suggest storing it at 4C after thawing out from -20C and adding a carrier and freezing in aliquots if long term storage and multiple use is anticipated. Please be aware that because of this initial freezing and thawing cycle, the activity of the protein might be lower than expected.

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Question

Could you please let me know if this code can be used in cell culture? Thanks in advance

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Answer

Thank you for your email. This product is suitable for cell culture experiments. Please let me know if you have further questions.

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