Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: HPLC, WB, Functional Studies, SDS-PAGE
Product nameRecombinant human TGF beta 3 protein (Active)
See all TGF beta 3 proteins and peptides
ab52313 is fully biologically active.
The ED50 was determined by cytotoxicity assay using the WHO standard 98/608 as a direct comparison is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 108 units/mg.
Purity> 98 % SDS-PAGE.
>98% HPLC analyses
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS
Predicted molecular weight26 kDa
Amino acids301 to 412
Additional sequence information(Gene symbol: TGFB3) (Gene ID: 7043)
Our Abpromise guarantee covers the use of ab52313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is manufactured by BioVision, an Abcam company and was previously called 4344 TGF-beta3, human recombinant. 4344-5 is the same size as the 5 µg size of ab52313.
Endotoxin level: <0.1 ng/μg.
Centrifuge the vial prior to opening.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Please see notes section.
Constituents: 20% Ethanol, 0.06% Acetic acid buffer
This product is an active protein and may elicit a biological response in vivo, handle with caution.
FunctionInvolved in embryogenesis and cell differentiation.
Involvement in diseaseDefects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab52313 has been referenced in 1 publication.
- Ye P Modulation of epithelial tight junctions by TGF-beta 3 in cultured oral epithelial cells. Aust Dent J 57:11-7 (2012). PubMed: 22369552