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    products/proteins-peptides/recombinant-human-tnfl9-protein-active-ab283931.pdf

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Bioactive grade

Recombinant Human TNFL9 Protein (Active) (ab283931)

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Functional Studies - Recombinant Human 4-1BB Ligand/TNFSF9 Protein (ab283931)
  • SDS-PAGE - Recombinant Human 4-1BB Ligand/TNFSF9 Protein (ab283931)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: >= 95% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human TNFL9 Protein (Active)
    See all 4-1BBL proteins and peptides
  • Biological activity

    Fully biologically active determined by the dose dependent proliferation of PBMCs. ED50 is ≤ 25 ng/mL, corresponding to a specific activity of 0.4x105 units/mg.

  • Purity

    >= 95 % SDS-PAGE.

  • Expression system

    HEK 293 cells
  • Accession

    P41273
  • Protein length

    Protein fragment
  • Animal free

    Yes
  • Carrier free

    Yes
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE
    • Predicted molecular weight

      20 kDa
    • Amino acids

      71 to 254
    • Additional sequence information

      N-terminal Glycine.

Specifications

Our Abpromise guarantee covers the use of ab283931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at Room Temperature. Store at Room Temperature.

    pH: 7.40
    Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

General Info

  • Alternative names

    • 4 1BB L
    • 4 1BB ligand
    • 4 1BBL
    • 4-1BB ligand
    • 4-1BBL
    • Cd137l
    • Cd157l
    • Homolog of mouse 4 1BB L
    • Homolog of mouse 4 1BBL
    • ILA ligand (TNF related)
    • Ly63l
    • Receptor 4 1BB ligand
    • TNF superfamily member 9
    • TNFL9_HUMAN
    • Tnfsf9
    • TNLG5A
    • Tumor necrosis factor (ligand) superfamily member 9
    • Tumor necrosis factor ligand 5A
    • Tumor necrosis factor ligand superfamily member 9
    • Tumor necrosis factor superfamily member 9
    see all
  • Function

    Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
  • Tissue specificity

    Expressed in brain, placenta, lung, skeletal muscle and kidney.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P41273 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant Human 4-1BB Ligand/TNFSF9 Protein (ab283931)
    Functional Studies - Recombinant Human 4-1BB Ligand/TNFSF9 Protein (ab283931)

    Fully biologically active determined by the dose dependent proliferation of PBMCs. ED50 is ≤ 25 ng/mL, corresponding to a specific activity of 0.4x105 units/mg.

    Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

    Lot GR3418469-2

  • SDS-PAGE - Recombinant Human 4-1BB Ligand/TNFSF9 Protein (ab283931)
    SDS-PAGE - Recombinant Human 4-1BB Ligand/TNFSF9 Protein (ab283931)

    SDS-PAGE analysis of ab283931.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab283931? Please let us know so that we can cite the reference in this datasheet.

ab283931 has not yet been referenced specifically in any publications.

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