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Recombinant Human TRAF6 protein (ab132023)

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SDS-PAGE - Recombinant Human TRAF6 protein (ab132023)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: SDS-PAGE, ELISA, WB

    Description

    • Product name

      Recombinant Human TRAF6 protein
      See all TRAF6 proteins and peptides

    • Expression system

      Wheat germ

    • Accession

      Q9Y4K3

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MSLLNCENSCGFSQSESDCCVAMASSCSAVTKDDSVGGTASTGNLSSSFM EEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRD AGHKCPVDNEILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLED HQAHCEFALMDCPQCQRPFQKFHINIHILKDCPRRQVSCDNCAASMAFED KEIHDQNCPLANVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCH EKMQRNHLARHLQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIH QLEGRLVRQDHQIRELTAKMETQSMYVSELKRTIRTLEDKVAEIEAQQCN GIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTA QRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIM DAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIKDDTLLVRCEVS TRFDMGSLRREGFQPRSTDAGV

      • Predicted molecular weight

        86 kDa

      • Amino acids

        1 to 522

      • Tags

        GST tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab132023 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      ELISA

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • E3 ubiquitin-protein ligase TRAF6
      • Interleukin 1 signal transducer
      • Interleukin-1 signal transducer
      • MGC 3310
      • MGC:3310
      • MGC3310
      • OTTHUMP00000232772
      • OTTHUMP00000232773
      • RING finger protein 85
      • RNF 85
      • RNF85
      • TNF receptor associated factor 6
      • TNF receptor-associated factor 6
      • TNF receptor-associated factor 6, E3 ubiquitin protein ligase
      • TRAF 6
      • Traf6
      • TRAF6_HUMAN
      see all

    • Function

      E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor.

    • Tissue specificity

      Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Belongs to the TNF receptor-associated factor family. A subfamily.
      Contains 1 MATH domain.
      Contains 1 RING-type zinc finger.
      Contains 2 TRAF-type zinc fingers.

    • Domain

      The coiled coil domain mediates homo- and hetero-oligomerization.
      The MATH/TRAF domain binds to receptor cytoplasmic domains.

    • Post-translational
      modifications

      Sumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
      Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form.

    • Cellular localization

      Cytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human TRAF6 protein (ab132023)
      SDS-PAGE - Recombinant Human TRAF6 protein (ab132023)
      12.5% SDS-PAGE stained with Coomassie Blue showing ab132023 at approximately 86 kDa.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab132023? Please let us know so that we can cite the reference in this datasheet.

    ab132023 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Submit a review Submit a question

    Question

    Does your recombinant TRAF6 have activity (E3 ligase)? What expression system did you use to generate this proteins?

    Read More
    Answer

    We have not tested the recombinant TRAF6 ab132023 in an activity assay. It may have activity but the only we have data are from the immunoassays listed on the datasheet, ELISA and western blotting. The expression system was wheat germ. The web page at the following link contains a basic introduction to this expression system.

    https://www.abcam.com/?pageconfig=resource&rid=14021

    Read More

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