Recombinant human TYK2 protein (ab125539)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human TYK2 protein -
Biological activity
The specific activity of ab125539 was determined to be 24 nmol/min/mg.
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Purity
> 70 % Densitometry.
Purity determined to be >70% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
110 kDa including tags -
Amino acids
442 to 1187
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab125539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- JTK 1
- JTK1
- Non receptor tyrosine protein kinase 2
see all -
Function
Probably involved in intracellular signal transduction by being involved in the initiation of type I IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain. -
Tissue specificity
Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines. -
Involvement in disease
Defects in TYK2 are the cause of protein-tyrosine kinase 2 deficiency (TYK2 deficiency) [MIM:611521]; also known as autosomal recessive hyper-IgE syndrome (HIES) with atypical mycobacteriosis. TYK2 deficiency consists of a primary immunodeficiency characterized by recurrent skin abscesses, pneumonia, and highly elevated serum IgE. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
Domain
The FERM domain mediates interaction with JAKMIP1. - Information by UniProt
Images
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SDS-PAGE - Recombinant human TYK2 protein (ab125539)SDS PAGE analysis of ab125539
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Functional Studies - Recombinant human TYK2 protein (ab125539)The specific activity of TYK2 (ab125539) was determined to be 20 nmol/min/mg as per activity assay protocol.
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SDS-PAGE - Recombinant human TYK2 protein (ab125539)SDS-PAGE analysis of ab125539. -
Functional Studies - Recombinant human TYK2 protein (ab125539)The specific activity of ab125539 was determined to be 24 nmol/min/mg by Kinase Assay.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125539 has not yet been referenced specifically in any publications.