Recombinant Human Vav proteins (ab172184)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human Vav proteins -
Purity
> 70 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
PFPTEEESVGDEDIYSGLSDQIDDTVEEDEDLYDCVENEEAEGDEIYEDL MRSEPVSMPPKMTEYDKRCCCLREIQQTEEKYTDTLGSIQQHFLKPLQRF LKPQDIEIIFINIEDLLRVHTHFLKEMKEALGTPGAANLYQVFIKYKERF LVYGRYCSQVESASKHLDRVAAAREDVQMKLEECSQRANNGRFTLRDLLM VPMQRVLKYHLLLQELVKHTQEAMEKENLRLALDAMRDLAQCVNEVKRDN ETLRQITNFQLSIENLDQSLAHYGRPKIDGELKITSVERRSKMDRYAFLL DKALLICKRRGDSYDLKDFVNLHSFQVRDDSSGDRDNKKWSHMFLLIEDQ GAQGYELFFKTRELKKKWMEQFEMAISNIYPENATANGHDFQMFSFEETT SCKACQMLLRGTFYQGYRCHRCRASAHKECLGRVPPCGRHGQDFPGTMKK DKLHRRAQDKKRNELGLPKMEVFQEYYGLPPPPGAIGPFLRLNPGDIVEL TKAEAEQNWWEGRNTSTNEIGWFPCNRVKPYVHGPPQDLSVHLWYAGPME RAGAESILANRSDGTFLVRQRVKDAAEFAISIKYNVEVKHIKIMTAEGLY RITEKKAFRGLTELVEFYQQNSLKDCFKSLDTTLQFPFKEPEKRTISRPA VGSTKYFGTAKARYDFCARDRSELSLKEGDIIKILNKKGQQGWWRGEIYG RVGWFPANYVEEDYSEYC -
Predicted molecular weight
120 kDa including tags -
Amino acids
73 to 845 -
Tags
GST tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab172184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 73% Tris-HCl buffer, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF
General Info
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Alternative names
- 2810040F13Rik
- A530094I06Rik
- AA986410
see all -
Relevance
The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response. -
Cellular localization
Cytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3
Images
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SDS-PAGE - Recombinant Human Vav proteins (ab172184)
ab172184 on SDS-PAGE, MW ~120 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab172184 has not yet been referenced specifically in any publications.