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Recombinant human VEGF Receptor 2 protein (ab42614)

Submit a review Q&A (6)

Key features and details

  • Expression system: Baculovirus infected insect cells
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Suitable for: Inhibition Assay

Description

  • Product name

    Recombinant human VEGF Receptor 2 protein
    See all VEGF Receptor 2 proteins and peptides

  • Biological activity

    40 U/µg. One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na-orthovanadate, 0.5 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate and 1 µg/ml recombinant VEGF Receptor 2.

  • Purity

    > 90 % SDS-PAGE.
    Affinity purified.

  • Expression system

    Baculovirus infected insect cells

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Amino acids

      805 to 1356

Specifications

Our Abpromise guarantee covers the use of ab42614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Inhibition Assay

  • Form

    Liquid

  • Additional notes

    This protein was expressed using a Baculovirus infected Sf9 cell expression system.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CD309
    • CD309 antigen
    • EC 2.7.10.1
    • Fetal liver kinase 1
    • FLK-1
    • FLK1
    • FLK1, mouse, homolog of
    • Kdr
    • Kinase insert domain receptor
    • Kinase insert domain receptor (a type III receptor tyrosine kinase)
    • KRD1
    • Ly73
    • Protein tyrosine kinase receptor FLK1
    • Protein-tyrosine kinase receptor flk-1
    • soluble VEGFR2
    • Tyrosine kinase growth factor receptor
    • Vascular endothelial growth factor receptor 2
    • VEGFR
    • VEGFR 2
    • VEGFR-2
    • VEGFR2
    • VGFR2_HUMAN
    see all

  • Function

    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

  • Involvement in disease

    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.

  • Cellular localization

    Membrane.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab42614? Please let us know so that we can cite the reference in this datasheet.

ab42614 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

Question

If the notes refer to the full length of protein, what the phosphorylation status of this product ab42614?

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Answer

I am sorry my previous email was unclear.

The phosphorylation status of ab42614 is no known. This is not something that is determined during the production process.

I hope this information is helpful.

Read More

Question



Thank you very much for your great suggestion. This product is perfect except the Agarose beads. Because we will use the centrifuge to separate the particle, the

rigidity ofAgarose may be not enough. However, I got very useful information from your email that I can use the polyclonal anti-GST antibody to fish out the GST-

tagged VEGFR protein. It is very important information for me. Thank you very much.



And another simple question is the phosphorylation of the product ab42614. From the datasheet, it says that ab42614 is “Phosphorylated. Dephosphorylated by

PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214”.



1, I just want to make sure whether all the 6 Tyrosine positions (Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214) are phosphorylated.



2, For the dephosphorylation, there are two products mentioned “PTPRB” and “PTPRJ”. I’d like to do the dephosphorylation at Tyr-951, Tyr-996, Tyr-1054, Tyr-

1059, Tyr-1175 and Tyr-1214, which item should I choose?

Read More
Answer

Thank you for your reply.

Actually it is fine to centrifuge agarose beads. Typically for IP I have centrifuged agarose beads at 500xg for 3-5 min at 4 degrees C.

Regarding your questions 1 and 2, those notes on the datasheet for ab42614 are general notes that just refer to the biochemical properties of the full length protein.

I hope this is helpful. Please feel free to contact me if you have further questions.

Read More

Question

Sorry to interrupt you again. I really got some further questions.



Actually, I want to choose an Anti-GST antibody as a “bait” to fish out the GST-tagged VEGFR. For the bait, I hope to immobilize the antibody to some particles. I find some Anti-GST antibody (Biotin) products on your website that I’m interested. My questions are:



1, What kind of GST-tag (ab42614) it belongs to, human, rabbit, goat or sth.? May I use the anti-GST antibody to immuno- fish out that GST-tagged VEGFR?



2, I found that all the Anti-GST antibody (Biotin) products on your websites are polyclonal. How about the immuno-affinity of polyclonal antibody to GST compared to the monoclonal one? May I use the polyclonal bait to fish out the GST-tagged VEGFR?



3, I’d like to use the biotin-group of Anti-GST antibody for its immobilization. Is it possible to use a streptavidin-modified particle to immobilize the Anti-GST antibody (Biotin)?



Sorry for asking so many questions. I’m looking forward your professional suggestions.

Read More
Answer

Thank you for your enquiry.

I think I have found a product that will suit your needs:

https://www.abcam.com/GST-antibody-Agarose-ab90667.html

This is anti-GST antibody covalently linked to agarose beads. Using this product would save you additional steps of first finding an anti-GST antibody and then locating an appropriate support.

I hope this is helpful. Please let me know if you have any further questions!

Read More

Question


Thanks a lot for your useful information. I appreciate it very much.

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Answer

I'm happy to help! Please contact me again if you need anything further.

Read More

Question

Hi,



I’m sorry to interrupt you. I have some questions about your product.



We just bought a VEGF Receptor 2 protein (Tagged) (ab42614) from your company. However, we can’t find the Tag information from the website. Because we want to utilize the tag for our further research, I just wonder what kind of tag is used for this protein.



I noticed another product we bought VEGF protein (His tag) (ab55566) giving the tag information. Could you help me to check what kind of proprietary tag of the ab42614 ?



Thank you for your great help!

Read More
Answer

Thank you for your enquiry.

VEGF Receptor 2 protein (Tagged) (ab42614) contains an N-terminal GST tag.

I hope this is helpful. Please contact me again if you have any further questions.

Read More

Question

I am interested in VEGF recepotr 2 protein (ab54347). I want to use it in a kinase assay. So I'd like to know whether this product has kinase activity?  

Read More
Answer

Thank you for your patience while I looked into your question. I have heard back from the lab, and ab106310 is also only the extracellular domain of VEGF receptor 2, and is thus not suitable for kinase studies. However, ab42614 has been tested for kinase activity and would be a good choice for your studies. The phosphorylation activity has been determined as 40 U/µg, where one unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. I hope this information is helpful, but please let me know if you have any questions.

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