Recombinant mouse Eph receptor B1/NET protein (ab56257)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant mouse Eph receptor B1/NET protein
See all Eph receptor B1/NET proteins and peptides -
Biological activity
Specific Activity: 143 nmol/min/mg.
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Purity
> 90 % SDS-PAGE.
Source: Sf9 insect cells. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Amino acids
591 to 984
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab56257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity.
Product previously known as Recombinant mouse Eph receptor B1 protein.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00154% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Cek 6
- EK6
- ELK
see all -
Function
Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. Binding with the guidance cue ephrin-B2 at the optic chiasm midline redirect ventrotemporal (VT) retinal ganglion cells (RGCs) axons ipsilaterally. May be involved in cell-cell interactions in the nervous system. -
Tissue specificity
Preferentially expressed in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant mouse Eph receptor B1/NET protein (ab56257)
SDS PAGE analysis of ab56257.
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Functional Studies - Recombinant mouse Eph receptor B1/NET protein (ab56257)The specific activity of Eph receptor B1/NET (ab56257) was determined to be 82 nmol /min/mg as per activity assay protocol.
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Functional Studies - Recombinant mouse Eph receptor B1/NET protein (ab56257)Kinase Assay Activity Plot.ab56257 at 0.1µg/µl.
Specific activity determined as 143 nmol/min/mg.
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SDS-PAGE - Recombinant mouse Eph receptor B1/NET protein (ab56257)ab56257 on SDS-PAGE, MW 62kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab56257 has not yet been referenced specifically in any publications.