Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant mouse M-CSF protein (Active)
See all M-CSF proteins and peptides
NFS-60 cell proliferation. ≤10 ng/mL; ≥ 1.0 x 105 units/mg.
Purity> 95 % SDS-PAGE.
Endotoxin level<=1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceMKEVSEHCSHMIGNGHLKVLQQLIDSQMETSCQIAFEFVDQEQLDDPVCY LKKAFFLVQDIIDETMRFKDNTPNANATERLQELSNNLNSCFTKDYEEQN KACVRTFHETPLQLLEKIKNFFNETKNLLEKDWNIFTKNCNNSFAKCSSR DVVTKP
Predicted molecular weight18 kDa
Amino acids33 to 187
Our Abpromise guarantee covers the use of ab256080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at room temperature. Store at -20°C.
Constituents: 0.16% Sodium phosphate, 0.58% Sodium chloride
0.2 micron filtered
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in sterile water at 0.1 mg/ml. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
- Colony stimulating factor 1
- Colony stimulating factor 1 (macrophage)
- Colony stimulating factor macrophage specific
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
modificationsGlycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab256080 has not yet been referenced specifically in any publications.