Recombinant Mouse PD-L1 protein (Fc Chimera) (Biotin) (ab216261)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Tags: Avi tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Mouse PD-L1 protein (Fc Chimera) (Biotin)
See all PD-L1 proteins and peptides -
Purity
> 90 % SDS-PAGE.
>90% purity as measured by gel filtration. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
AFTITAPKDLYVVEYGSNVTMECRFPVERELDLLALVVYWEKEDEQVIQF VAGEEDLKPQHSNFRGRASLPKDQLLKGNAALQITDVKLQDAGVYCCIIS YGGADYKRITLKVNAPYRKINQRISVDPATSEHELICQAEGYPEAEVIWT NSDHQPVSGKRSVTTSRTEGMLLNVTSSLRVNATANDVFYCTFWRSQPGQ NHTAELIIPELPATHPPQNRT -
Predicted molecular weight
54 kDa including tags -
Amino acids
18 to 238 -
Tags
Avi tag C-Terminus -
Additional sequence information
Fused at the C-terminus to the Fc portion of mouse IgG2a (NM_021893). Biotinylated using AviTag technology.
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Conjugation
Biotin
Specifications
Our Abpromise guarantee covers the use of ab216261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 79% Phosphate Buffer
General Info
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Alternative names
- B7 H
- B7 H1
- B7 homolog 1
see all -
Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane and Endomembrane system. - Information by UniProt
Images
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SDS-PAGE - Recombinant Mouse PD-L1 protein (Fc Chimera) (Biotin) (ab216261)
4-20% SDS-PAGE analysis of 3 μg ab216261.
This protein runs at an apparent higher molecular weight due to glycosylation.
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Size Exclusion Chromatography - Recombinant Mouse PD-L1 protein (Fc Chimera) (Biotin) (ab216261)Gel filtration curve of ab216261.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab216261 has been referenced in 2 publications.
- Kong F et al. PD-L1 Improves Motor Function and Alleviates Neuropathic Pain in Male Mice After Spinal Cord Injury by Inhibiting MAPK Pathway. Front Immunol 12:670646 (2021). PubMed: 33936116
- Liu BL et al. Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia. JCI Insight 5:N/A (2020). PubMed: 32960817