Recombinant Mouse PGP9.5 protein (ab202233)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
Description
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Product name
Recombinant Mouse PGP9.5 protein
See all PGP9.5 proteins and peptides -
Purity
> 90 % SDS-PAGE.
Purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMQLKPMEINPEMLNKVLAKLGVAGQWR FADVLGLEEETLGSVPSPACALLLLFPLTAQHENFRKKQIEELKGQEVSP KVYFMKQTIGNSCGTIGLIHAVANNQDKLEFEDGSVLKQFLSETEKLSPE DRAKCFEKNEAIQAAHDSVAQEGQCRVDDKVNFHFILFNNVDGHLYELDG RMPFPVNHGASSEDSLLQDAAKVCREFTEREQGEVRFSAVALCKAA -
Predicted molecular weight
27 kDa including tags -
Amino acids
1 to 223 -
Tags
His tag N-Terminus -
Additional sequence information
NP_035800.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab202233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 89% PBS, 0.02% DTT, 10% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Epididymis luminal protein 117
- Epididymis secretory protein Li 53
- HEL 117
see all -
Function
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
Tissue specificity
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
Involvement in disease
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
Sequence similarities
Belongs to the peptidase C12 family. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
Images
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SDS-PAGE - Recombinant Mouse PGP9.5 protein (ab202233)
15% SDS-PAGE analysis of ab202233 (3µg).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab202233 has not yet been referenced specifically in any publications.