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Recombinant mouse PKM2 protein (Active) (ab223006)

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SDS-PAGE - Recombinant mouse PKM2 protein (Active) (ab223006)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: Functional Studies, SDS-PAGE, MS

    Description

    • Product name

      Recombinant mouse PKM2 protein (Active)
      See all PKM2 proteins and peptides

    • Biological activity

      Specific activity: > 50,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C.

    • Purity

      > 85 % SDS-PAGE.
      ab223006 was urified by using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      P52480-1

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Mouse

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMGSMPKPHSEAGTAFIQTQQLHAAMADTFL EHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFS HGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGS GTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVD DGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQ DLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGV RRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICA TQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRM QHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGA IIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDA VLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRV VPVP

      • Predicted molecular weight

        60 kDa including tags

      • Amino acids

        1 to 531

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab223006 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.50
      Constituents: 0.32% Tris HCl, 1.16% Sodium chloride, 0.02% DTT, 30% Glycerol (glycerin, glycerine)

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • CTHBP
      • Cytosolic thyroid hormone binding protein
      • Cytosolic thyroid hormone-binding protein
      • KPYM_HUMAN
      • MGC3932
      • OIP 3
      • OIP-3
      • OIP3
      • OPA interacting protein 3
      • Opa-interacting protein 3
      • p58
      • PK muscle type
      • PK, muscle type
      • PK2
      • PK3
      • PKM
      • PKM2
      • pykm
      • Pyruvate kinase 2/3
      • Pyruvate kinase 3
      • Pyruvate kinase isozymes M1/M2
      • Pyruvate kinase muscle
      • Pyruvate kinase muscle isozyme
      • pyruvate kinase PKM
      • Pyruvate kinase, muscle 2
      • TCB
      • THBP1
      • Thyroid hormone binding protein 1
      • Thyroid hormone binding protein cytosolic
      • Thyroid hormone-binding protein 1
      • Tumor M2 PK
      • Tumor M2-PK
      see all

    • Function

      Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

    • Tissue specificity

      Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.

    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

    • Sequence similarities

      Belongs to the pyruvate kinase family.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
      ISGylated.

    • Cellular localization

      Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant mouse PKM2 protein (Active) (ab223006)
      SDS-PAGE - Recombinant mouse PKM2 protein (Active) (ab223006)

      15% SDS-PAGE - Recombinant mouse PKM2 protein (Active) (3 µg ab223006).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab223006? Please let us know so that we can cite the reference in this datasheet.

    ab223006 has not yet been referenced specifically in any publications.

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