Recombinant Proteinase K (Active) (ab281339)
Key features and details
- Expression system: Yeast
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 0.250 Eu/mg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant Proteinase K (Active)
See all Proteinase K proteins and peptides
Specific activity >30 units/mg.
Purity>= 95 % SDS-PAGE.
Endotoxin level< 0.250 Eu/mg
Protein lengthFull length protein
SequenceAAQTNAPWGL ARISSTSPGT STYYYDESAG QGSCVYVIDT GIEASHPEFE GRAQMVKTYY YSSRDGNGHG THCAGTVGSR TYGVAKKTQL FGVKVLDDNG SGQYSTIIAG MDFVASDKNN RNCPKGVVAS LSLGGGYSSS VNSAAARLQS SGVMVAVAAG NNNADARNYS PASEPSVCTV GASDRYDRRS SFSNYGSVLD IFGPGTSILS TWIGGSTRSI SGTSMATPHV AGLAAYLMTL GKTTAASACR YIADTANKGD LSNIPFGTVN LLAYNNYQA
Predicted molecular weight29 kDa including tags
Amino acids106 to 384
TagsHis tag N-Terminus
Additional sequence informationTritirachium album (Engyodontium album). Full-length mature chain lack the signal and pro-peptides.
Our Abpromise guarantee covers the use of ab281339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: 6% Trehalose, 0.32% Tris HCl
Buffer lyophilized from.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.
- Endopeptidase K
- Tritirachium alkaline proteinase
RelevanceProteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name. Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
ab281339 has not yet been referenced specifically in any publications.