Recombinant Proteinase K (Active) (ab281339)
Key features and details
- Expression system: Yeast
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 0.250 Eu/mg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant Proteinase K (Active)
See all Proteinase K proteins and peptides -
Biological activity
Specific activity >30 units/mg.
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Purity
>= 95 % SDS-PAGE. -
Endotoxin level
< 0.250 Eu/mg -
Expression system
Yeast -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Nature
Recombinant -
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Sequence
AAQTNAPWGL ARISSTSPGT STYYYDESAG QGSCVYVIDT GIEASHPEFE GRAQMVKTYY YSSRDGNGHG THCAGTVGSR TYGVAKKTQL FGVKVLDDNG SGQYSTIIAG MDFVASDKNN RNCPKGVVAS LSLGGGYSSS VNSAAARLQS SGVMVAVAAG NNNADARNYS PASEPSVCTV GASDRYDRRS SFSNYGSVLD IFGPGTSILS TWIGGSTRSI SGTSMATPHV AGLAAYLMTL GKTTAASACR YIADTANKGD LSNIPFGTVN LLAYNNYQA -
Predicted molecular weight
29 kDa including tags -
Amino acids
106 to 384 -
Tags
His tag N-Terminus -
Additional sequence information
Tritirachium album (Engyodontium album). Full-length mature chain lack the signal and pro-peptides.
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Specifications
Our Abpromise guarantee covers the use of ab281339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 6% Trehalose, 0.32% Tris HCl
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.
General Info
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Alternative names
- Endopeptidase K
- Tritirachium alkaline proteinase
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Relevance
Proteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name. Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes.
Images
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SDS-PAGE - Recombinant Proteinase K (Active) (ab281339)
SDS-PAGE analysis of ab281339. 15% gel. Reducing conditions.
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Functional Studies - Recombinant Proteinase K (Active) (ab281339)Specific activity >30 Units/mg.
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Functional Studies - Recombinant Proteinase K (Active) (ab281339)Agarose gel analysis of residual nucleic acid in ab281339.
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Functional Studies - Recombinant Proteinase K (Active) (ab281339)Agarose gel analysis of RNAase activity in ab281339.
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Functional Studies - Recombinant Proteinase K (Active) (ab281339)Agarose gel analysis of DNAase activity in ab281339.
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Functional Studies - Recombinant Proteinase K (Active) (ab281339)Functional analysis of ab281339.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab281339 has not yet been referenced specifically in any publications.
