Recombinant Staphylococcus aureus Protein A (ab52953)
Key features and details
- Expression system: Escherichia coli
- Purity: > 99% SDS-PAGE
- Endotoxin level: < 0.100 Eu/mg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
-
Product name
Recombinant Staphylococcus aureus Protein A
See all Protein A proteins and peptides -
Purity
> 99 % SDS-PAGE.
Purity: >99% by SDS-PAGE and HPLC analyses. -
Endotoxin level
< 0.100 Eu/mg -
Expression system
Escherichia coli -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Staphylococcus aureus -
Predicted molecular weight
36 kDa -
Amino acids
32 to 327 -
Tags
His tag N-Terminus -
Additional sequence information
Protein A Ig binding domains with 6x His-tag on N-terminus. There is a linker between the His tag and recombinant fragment (~11 aa). The sequence cannot be shared.
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab52953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Liquid -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 6500 Protein A (Liquid form). 6500-10 is the same size as the 10 mg size of ab52953.
The cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from this protein to ensure the maximum specific IgG binding. . The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies.
Protein A binds to most human and mouse IgG subclasses (e.g: human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).
It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit.
Protein A has little affinity to chicken, goat, rat and sheep. -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: Water
General Info
-
Alternative names
- gG-binding protein A
- Immunoglobulin G binding protein A
- SPA
- Staphylococcal protein A
-
Relevance
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
Cellular localization
Secreted; cell wall.
Images
-
SDS-PAGE - Recombinant Protein A (ab52953)
SDS-PAGE (4-20%) of Recombinant Protein A.
Lane 1-3: 5, 2.5, and 1 µg, Protein A under Reducing conditions,
Lane4: protein Ladder;
Lane5-7: 5, 2.5, and 1 µg of Protein A under Non-Reducing conditions
-
Size Exclusion Chromatography - Recombinant Protein A (ab52953)SEC chromatography of Protein A. The apparent molecular weight is ~ 70 KDa calculated based on SEC standard curve.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (2)
ab52953 has been referenced in 2 publications.
- Sharafeldin M et al. Influence of antibody immobilization strategy on carbon electrode immunoarrays. Analyst 144:5108-5116 (2019). PubMed: 31373337
- Kim JJ et al. Microparticle Delivery of Protein Markers for Single-Cell Western Blotting from Microwells. Small 14:e1802865 (2018). PubMed: 30334351
