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Recombinant X. laevis PKC alpha protein (ab60839)

Submit a review Q&A (1)
Functional Studies - Recombinant <em>X. laevis</em> PKC alpha protein (ab60839)
  • SDS-PAGE - Recombinant <em>X. laevis</em> PKC alpha protein (ab60839)
  • SDS-PAGE - Recombinant <em>X. laevis</em> PKC alpha protein (ab60839)
  • Functional Studies - Recombinant <em>X. laevis</em> PKC alpha protein (ab60839)
  • SDS-PAGE - Recombinant <em>X. laevis</em> PKC alpha protein (ab60839)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 80% Densitometry
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant X. laevis PKC alpha protein
    See all PKC alpha proteins and peptides

  • Purity

    > 80 % Densitometry.
    Affinity purified.

  • Expression system

    Baculovirus infected Sf9 cells

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Xenopus laevis

  • Description

    Recombinant X. laevis PKC alpha protein

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • AAG6
    • Aging associated gene 6
    • aPKC
    • KPCA_HUMAN
    • PKC alpha
    • PKC-A
    • PKC-alpha
    • PKCA
    • PRKACA
    • PRKCA
    • Protein Kinase C alpha
    • Protein kinase C alpha type
    see all

  • Function

    This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.

  • Cellular localization

    Cytoplasm. Cell membrane. Nucleus.
  • Information by UniProt

Images

  • Functional Studies - Recombinant <em>X. laevis</em> PKC alpha protein (ab60839)
    Functional Studies - Recombinant X. laevis PKC alpha protein (ab60839)
    The specific activity of PKC alpha (ab60839) was determined to be 250 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant <em>X. laevis</em> PKC alpha protein (ab60839)
    SDS-PAGE - Recombinant X. laevis PKC alpha protein (ab60839)
    SDS PAGE analysis of ab60839
  • SDS-PAGE - Recombinant <em>X. laevis</em> PKC alpha protein (ab60839)
    SDS-PAGE - Recombinant X. laevis PKC alpha protein (ab60839)
    SDS PAGE analysis of ab60839
  • Functional Studies - Recombinant <em>X. laevis</em> PKC alpha protein (ab60839)
    Functional Studies - Recombinant X. laevis PKC alpha protein (ab60839)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant <em>X. laevis</em> PKC alpha protein (ab60839)
    SDS-PAGE - Recombinant X. laevis PKC alpha protein (ab60839)
    ab60839 on SDS-PAGE, MW ~103 kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab60839? Please let us know so that we can cite the reference in this datasheet.

ab60839 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

I ordered PKC alpha protein (Active).(ab60839, Serial no: k1111-900) from your company, and want to use it to phosphorylate an E.Coli purified protein, but I could not find the protocol or the recipe of kinase reaction buffer for this kinase on your website. I also found there are different recipe of kinase reaction buffer for PKC alpha protein on the web. So could you suggest a standard protocol or a recipe of kinase reaction buffer for this kinase for phosphorylating a E.Coli purified protein?

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Answer

Thank you for contacting us.

Please find attached a modified version of the radiolabel (phosphorus 33-ATP) protocol that was used to measure activity of ab60839. The assay utilized CREBtide as the substrate (KRREILSRRPSYR) but the lab believes you can simply substitute your E. coli protein for the peptide, which I have noted in bold at the bottom of the materials section of the protocol.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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