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    products/proteins-peptides/streptavidin-hrp-ab7403.pdf

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Streptavidin (HRP) (ab7403)

  • Datasheet
  • SDS
Reviews (6)Q&A (14)References (121)

Product price, shipping and contact information

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (ab7403)
  • ELISA - Streptavidin (HRP) (ab7403)

Key features and details

  • Expression system: Native
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC, WB

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Description

  • Product name

    Streptavidin (HRP)
    See all Streptavidin proteins and peptides
  • Biological activity

    Binds to Biotin. Dissociation constant has not been measured.

  • Purity

    > 95 % SDS-PAGE.
    Chromatographically pure, a single band by SDS-PAGE. Streptavidin-HRP was prepared from chromatographically purified streptavidin.  Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.
  • Expression system

    Native
  • Accession

    P22629
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Streptomyces avidinii
    • Sequence

      MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLG STFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWT VAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVG HDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ
  • Conjugation

    HRP
  • Description

    Native Streptavidin protein (HRP)

Associated products

  • Substrate reagent

    • ABTS™, Peroxidase substrate (ab142041)

Specifications

Our Abpromise guarantee covers the use of ab7403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Dot blot

    ELISA

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Frozen sections)

    Immunomicroscopy

    Immunocytochemistry

    Western blot

  • Form

    Liquid
  • Additional notes

    Horseradish peroxidase is conjugated to the streptavidin tetramer at ~1:1 molar ratio.

    This product has been assayed against 1.0 µg of Biotinylated IgG in a standard capture ELISA using a peroxidase substrate as ABTS ab142041 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.424% Potassium phosphate, 0.87% Sodium chloride, BSA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all
  • Relevance

    Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • Cellular localization

    Cytoplasmic

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (ab7403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (ab7403)

    IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab11080, 1/1000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ELISA - Streptavidin (HRP) (ab7403)
    ELISA - Streptavidin (HRP) (ab7403)

    The wells were coated with ab200699 at 1µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG3 (ab138703) was then added starting at 20 µg/ml and plasma/serum at 1:500 and gradually diluted 1:4, 50µl/well for 2h. Ab201248 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (121)

Publishing research using ab7403? Please let us know so that we can cite the reference in this datasheet.

ab7403 has been referenced in 121 publications.

  • Zhang Y  et al. An antibody-based proximity labeling map reveals mechanisms of SARS-CoV-2 inhibition of antiviral immunity. Cell Chem Biol 29:5-18.e6 (2022). PubMed: 34672954
  • Yaghoubi A  et al. Prednisolone and mesenchymal stem cell preloading protect liver cell migration and mitigate extracellular matrix modification in transplanted decellularized rat liver. Stem Cell Res Ther 13:36 (2022). PubMed: 35090559
  • Xu Z  et al. Induction of tier-2 neutralizing antibodies in mice with a DNA-encoded HIV envelope native like trimer. Nat Commun 13:695 (2022). PubMed: 35121758
  • Zhang G  et al. Dynamic FMR1 granule phase switch instructed by m6A modification contributes to maternal RNA decay. Nat Commun 13:859 (2022). PubMed: 35165263
  • Pinkaew D  et al. Fortilin interacts with TGF-ß1 and prevents TGF-ß receptor activation. Commun Biol 5:157 (2022). PubMed: 35197550
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 20 Abreviews or Q&A

Works good for staining

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA fixed)
Streptavidin HRP (1:1000) for IHC. Clear signal, no background.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 07 2021

Biotinylated protein detection ELISA

Excellent
Abreviews
Abreviews
abreview image
Application
ELISA
Capture : anti-target protein
Sample : Recombinant purified protein
1st Detection : Biotinylated anti-target protein
2nd Detection : Streptavidin HRP (1:4000)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 17 2020

WB for transfected biotinylated protein

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
I performed western blot with biotinylated proteins (BioID-tagged) transfected into HEK293T cells. The results clearly show a band at the expected molecular weight. Streptavidin (HRP) was used at 1:500 dilution in TBST with 2.5% BSA and incubated with the membrane 2 hours at room temperature.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 07 2020

Northern blot

Excellent
Abreviews
Abreviews
abreview image
Application
Other - Do not use
The Biotin-labeled probes for RNA U6 were detected using streptavidin-HRP.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 21 2020

Modified Biotin Switch assay for sulfhydration detection

Excellent
Abreviews
Abreviews
Application
Western blot
We performed modified biotin switch assay where the biotinylated sulfhydrated proteins were incubated with ab7403 - Streptavidin (HRP) for 1h at room temperature (1:100) and then immunoblotted for detection of sulfhydrated protein status.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 14 2018

Anti-CD9 sandwich ELISA

Excellent
Abreviews
Abreviews
abreview image
Application
ELISA
A standard sandwich ELISA experiment worked very well by use of ab7403 with 1/30,000 dilution.

Detailed protocol was described below.

*5 uL of human serum was separated with size exclusion chromatography into 10 fractions.
*1st antibody : anti-CD9 (Ancell Corporation)
*Blocking : 5% BSA in PBS
*2nd antibody : Biotinylated anti-CD9 (Ancell Corporation)

*HRP label : ab7403 - Streptavidin HRP, 1/30,000 dilution in 1% BSA/PBS, 30 min, RT

*1-Step Ultra TMB-ELISA Substrate Solution - Pierce
*Measure OD 450 nm
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 20 2014

Question

Besten Dank für Ihre Antwort, hier noch eine Anschlussfrage.
Wir sind dabei eine Methode zu entwickeln bei der potentiell folgende abcam Produkte involviert sein können: ab34531, Ab34572, ab34566 und ab7403.
Könnten Sie mir bitte eine Einschätzung betreffend der Langzeitverfügbarkeit dieser Produkte zuschicken.
Besten Dank und freundliche Grüsse

Read More

Abcam community

Verified customer

Asked on Nov 15 2012

Answer

Vielen Dank für Ihre Anfrage.
Leider kann ich Ihnen nicht garantieren, dass diese Produkte für immer in unserem Katalog bleiben. Diese Entscheidung hängt von vielen Faktoren ab und wird von verschiedenen Abteilungen wie Marketing, Produktion etc. getroffen. Ich kann Ihnen aber versichern, dass im Moment keine Pläne bestehen, diese Produkte aus dem Katalog zu nehmen.
Ich schlage vor diese vier Produkte zu testen. Wenn Sie Ihren Anforderungen entsprechen, besteht die Möglichkeit eine größere Menge (Bulk) zu bestellen. Diese kann auch als Lyophilisat geliefert werden und wäre somit für Jahre ohne Qualitätsverlust haltbar.
Ich hoffe, diese Information ist hilfreich und verbleibe

Read More

Abcam Scientific Support

Answered on Nov 15 2012

Question

Is it possible to order just the Streptavidin component of this kit?

Read More

Abcam community

Verified customer

Asked on Sep 12 2014

Answer

I am sorry, but the components found in ab177848 are not separately available.
However, we have a very similar reagent sold as a standalone product, Streptavidin HRP (ab7403) https://www.abcam.com/streptavidin-hrp-ab7403.html.
It may take a bit of optimization to determine the optimal dilution to use in your ELISA, but I recommend starting at 1:10,000 or 1:15,000 fold dilution.

Read More

Jeremy Kasanov

Abcam Scientific Support

Answered on Sep 12 2014

Question

Product code: 7403
Lot number: GR80435-9
Inquiry: This product arrived at our lab and the vial was stored directly at -20C. Being new to this product I am unsure about how it should be stored. The data sheet tells me to store it at 4C prior to reconstitution and thereafter store it at -20C, but the sheet also mentions that the state of the product is liquid. Would it be best to thaw the vial, aliquot it, and then again store the aliquots at -20C?

Read More

Abcam community

Verified customer

Asked on May 14 2014

Answer

Please thaw the content in tube and store it at 4C, it will be ok for several months (10-12 months). However if you want to store it for years then please aliquot it into smaller volume and then store it at -20C.

Read More

Padamjeet Singh

Abcam Scientific Support

Answered on May 14 2014

Question

Hello, I was wondering whether there is an antibody pair available for the ELISPOT detection of human CCL1/I-309. As far as I can see nothing is sold as such, but has it been tried or is it known whether maybe a sandwich elisa pair works for this purpose?

Read More

Abcam community

Verified customer

Asked on Dec 26 2012

Answer

I have confirmed that we do do not have an antibody pair tested for ELISPOT but we do have a pair, ab109788 and ab83413, that have been tested together in sandwich ELISA. Both are rabbit polyclonal antibodies raised against full-length recombinant human I-309, but one is biotinylated, which allows the two rabbit antibodies to be used together in ELISA. Detection is achieved with an avidin or streptavidin enzyme conjugate such as the HRP -conjugated streptavidin ab7403.

Efficacy in ELISPOT is assumed to be likely if efficacy in standard sandwich ELISA has been demonstrated, but optimizing the amount of capture antibody and detection antibody will be necessary.

There are other pairs that might work but that have not been tested together for ELISA of any kind. For example, the mouse monoclonal ab89446 could be used for capture, followed by detection with a polyclonal antibody raised against full-length protein, such as the rabbit anti-I-309 ab109788 or the goat anti-I-309 ab10377, followed by an anti-rabbit IgG or anti-goat IgG secondary conjugate that has been absorbed/purified to remove reactivity with mouse IgG. Please note that our guarantee only applies to tested applications.

Read More

Abcam Scientific Support

Answered on Dec 26 2012

1-10 of 20 Abreviews or Q&A

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