10X Annexin V/ANXA5 Binding Buffer (ab14084)
Overview
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Product name
10X Annexin V/ANXA5 Binding Buffer
See all Annexin V/ANXA5 reagents -
Tested applications
Suitable for: Flow Cyt, FMmore details -
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 1006 10X Annexin V Binding Buffer. 1006-100 is the same size as the 100 ml size of ab14084.
The binding buffer contains optimal concentration of calcium which is required for Annexin v/ANXA5 binding to phosphotidylserine (PS) on the cell surface. This 10X reagent should be diluted to 1X with distilled water (ddH2O) before using in the assay.
This buffer can be used in conjunction with our Annexin V/ANXA5 reagents.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
pH: 7.40
Concentrated x10 -
Concentration information loading...
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Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all
Associated products
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Related Products
- Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
- Propidium Iodide (ab14083)
- Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
- Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
- Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
- Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
- Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
- Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
- Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
- Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
- Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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FM |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
FM
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab14084 has been referenced in 4 publications.
- Nof E et al. Ventilation-induced epithelial injury drives biological onset of lung trauma in vitro and is mitigated with prophylactic anti-inflammatory therapeutics. Bioeng Transl Med 7:e10271 (2022). PubMed: 35600654
- Kochetkova EY et al. Approaches and Protocols to Analyze Autophagy and Its Role in Death of Apoptosis-Resistant Senescent Tumor Cells. Methods Mol Biol 2445:139-169 (2022). PubMed: 34972991
- Paez-Mayorga J et al. Ponatinib Activates an Inflammatory Response in Endothelial Cells via ERK5 SUMOylation. Front Cardiovasc Med 5:125 (2018). PubMed: 30238007
- Grant R et al. A filtration-based protocol to isolate human Plasma Membrane-derived Vesicles and exosomes from blood plasma. J Immunol Methods 371:143-51 (2011). PubMed: 21741384