10X Blocking Buffer (ab126587)
Overview
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Product name
10X Blocking Buffer -
Tested applications
Suitable for: WB, ELISA, In-Cell ELISA, ICC/IFmore details
Unsuitable for: Flow Cyt -
General notes
Protein blocking buffer (pH 7.5) (ab126587) for serum-free blocking of non-specific antibody binding in ICC, ELISA and western blot. BSA-free
Preparation: Dilute to 1X in deionized water or PBS (e.g. 1mL 10X blocking buffer + 9mL water/PBS). Diluting in PBS instead of water makes a somewhat more stringent blocking buffer due to the presence of salt in the PBS. Blocking Buffer is pH 7.5. The user should determine empirically whether dilution in water or PBS is most appropriate for the assay at hand. Use diluted block solution promptly.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at Room Temperature. The product can be stored for up to 12 months. -
Storage buffer
Constituent: Proprietary component -
Concentration information loading... -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
|
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| In-Cell ELISA |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
In-Cell ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
Images
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10X Blocking Buffer (ab126587)
Primary :
All Lanes : top membranes with Anti AMPK pThr172 + Thr183 (ab133448) at 1/1000. Bottom membranes with Anti AMPKa (ab110036) at 1/1000
Lane 1 = AMPKa1 protein
Lane 2 = C2C12 cells treated with AICAR
Lane 3 = untreated C2C12 cells
Secondary: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) and Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1:10,000 developed using the ECL technique.
Performed under reducing conditions (50mM DTT – Sample heated at 60ºC). Predicted band size for AMPKa: 64 kDa Observed band size for AMPKa: 64 kDa
Left membrane All blocking steps = 1X blocking reagent ab126587 diluted in PBST
Right membrane All blocking steps = 5% Milk in PBST Exposure time : 5 minutes
Primary :
All Lanes : top membranes with Anti AMPK pThr172 + Thr183 (ab133448) at 1/1000. Bottom membranes with Anti AMPKa (ab110036) at 1/1000
Lane 1 = AMPKa1 protein
Lane 2 = C2C12 cells treated with AICAR
Lane 3 = untreated C2C12 cells
Secondary: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) and Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1:10,000 developed using the ECL technique.
Performed under reducing conditions (50mM DTT – Sample heated at 60ºC). Predicted band size for AMPKa: 64 kDa Observed band size for AMPKa: 64 kDa
Left membrane All blocking steps = 1X blocking reagent ab126587 diluted in PBST
Right membrane All blocking steps = 5% Milk in PBST Exposure time : 5 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab126587 has been referenced in 18 publications.
- Yang JM et al. Gradient Waveguide Thickness Guided-Mode Resonance Biosensor. Sensors (Basel) 21:N/A (2021). PubMed: 33430392
- Dang TM et al. Optimization of the isolation procedure and culturing conditions for hepatic stellate cells obtained from mouse. Biosci Rep 41:N/A (2021). PubMed: 33350435
- Yokoe I et al. Lipid bubbles combined with low-intensity ultrasound enhance the intratumoral accumulation and antitumor effect of pegylated liposomal doxorubicin in vivo. Drug Deliv 28:530-541 (2021). PubMed: 33685314
- Ho CNQ et al. Simulated Microgravity Inhibits the Proliferation of Chang Liver Cells by Attenuation of the Major Cell Cycle Regulators and Cytoskeletal Proteins. Int J Mol Sci 22:N/A (2021). PubMed: 33925309
- Chou LY et al. Discoidin Domain Receptor 1 Regulates Runx2 during Osteogenesis of Osteoblasts and Promotes Bone Ossification via Phosphorylation of p38. Int J Mol Sci 21:N/A (2020). PubMed: 33003599