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10x EDTA Buffer pH 8.0 (ab64216)

Submit a review Q&A (3)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 10x EDTA Buffer pH 8.0 (ab64216)

    Overview

    • Product name

      10x EDTA Buffer pH 8.0

    • Tested applications

      Suitable for: IHC-Pmore details

    • General notes

      EDTA buffer pH 8.0 for heat-induced antigen retrieval (HIER) during IHC (ab64216).

      Recommended Procedure: Microwave

      1. Deparaffinize and rehydrate tissue sections.
      2. Dilute the product 1:10 with deionized water.
      3. Immerse sections in coplin jar filled with target retrieval solution microwave on HIGH power until boiling.
      4. Keep the slides warm by heating for 10min at LOW power.
      5. Let coplin jar sit in microwave for at least 20min.
      6. Remove and rinse with buffer.
      7. Proceed with staining.

    Properties

    • Form

      Liquid

    • Storage instructions

      Store at +4°C.

    • Storage buffer

      pH: 7.7
      Constituent: 0.29% EDTA

      Buffer 10x concentrated.
    • Concentration information loading...

    • Research areas

    • Relevance

      EDTA buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies.

    • Alternative names

      • Ethylenediaminetetraacetic acid buffer

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab64216 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use at an assay dependent dilution.
    Notes
    IHC-P
    Use at an assay dependent dilution.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 10x EDTA Buffer pH 8.0 (ab64216)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 10x EDTA Buffer pH 8.0 (ab64216)
      ab64216 Human Melanoma stained with an anti-S-100 antibody diluted with Antibody Diluent (ab64211)

    References (2)

    Publishing research using ab64216? Please let us know so that we can cite the reference in this datasheet.

    ab64216 has been referenced in 2 publications.

    • Posta N  et al. Hemoglobin oxidation generates globin-derived peptides in atherosclerotic lesions and intraventricular hemorrhage of the brain, provoking endothelial dysfunction. Lab Invest 100:986-1002 (2020). PubMed: 32054994
    • Hoang JD  et al. Cardiac sympathetic activation circumvents high-dose beta blocker therapy in part through release of neuropeptide Y. JCI Insight 5:N/A (2020). PubMed: 32493842

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Question

    Hi

    I have bought product (ab64216) but couldn’t find on the datasheet his molarity .

    Can you please help me if this information?

    Regards

    Read More
    Answer

    Thank you for your enquiry and patience.

    I have checked with my colleagues and the concentration is 10 mM EDTA. We will update the datasheet accordingly very soon.

    I apologize for any inconvenience caused and thank you for bringing this matter to our attention.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    Hope you are doing well !

    Please see below an e-mail, we need to do complete Rabies IHC test... i.e all materials from Antibody diluent to retrival to everything..


    Please advice me ASAP.. Also the customer needs specific protocol, as she is very new to IHC.

    Read More
    Answer

    Thank you for contacting us.

    Here is the list with the requested items. However, please be aware that this is not a technical inquiry as all item could be found by using the search application on our website under the "reagents" category. Please let us know if you need help on navigating our website so you can help your customers faster than relying on us.


    Regarding the Rabies antibody, I am afraid we do not have what your customer requests: All we have are mouse monoclonal antibodies against this virus, but none produced in goat. Please let me know if this expectable for her, so I can suggest the right primary antibody with the matching biotinylated secondary antibody.


    https://www.abcam.com/10x-Citrate-Buffer-pH-60-ab64214.html (or use the following: https://www.abcam.com/10x-Citrate-Buffer-pH-60-ab64214.html).

    https://www.abcam.com/10x-EDTA-Buffer-pH-80-ab64216.html (or use the following: https://www.abcam.com/10x-EDTA-Buffer-pH-80-ab64216.html).

    https://www.abcam.com/DAB-Substrate-Kit-ab64238.html (or use the following: https://www.abcam.com/DAB-Substrate-Kit-ab64238.html).

    https://www.abcam.com/Streptavidin-HRP-ready-to-use-ab64269.html (or use the following: https://www.abcam.com/Streptavidin-HRP-ready-to-use-ab64269.html).

    https://www.abcam.com/Hydrogen-Peroxide-Blocking-Reagent-ab94666.html (or use the following: https://www.abcam.com/Hydrogen-Peroxide-Blocking-Reagent-ab94666.html).

    https://www.abcam.com/Protein-Block-ab128988.html (or use the following: https://www.abcam.com/Protein-Block-ab128988.html).

    https://www.abcam.com/Mayers-Hematoxylin-ab128990.html (or use the following: https://www.abcam.com/Mayers-Hematoxylin-ab128990.html).



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Good afternoon We have purchased 10x EDTA Buffer pH 8.0 (ab64216) antigen retrieval.  I may be missing it on the spec sheet…..could you please tell me what mM it is?  I have been given a protocol calling for 10mM EDTA and I’m not sure if I can use this one.  

    Read More
    Answer

    ab64216 is supplied with a 10 mM solution of EDTA which is a 10X stock solution. The working concentration is 1mM EDTA. I apologize for any confusion. Please do not hesitate to contact me with additional questions or concerns.

    Read More

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