10X RIPA Buffer (ab156034)
Overview
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Product name
10X RIPA Buffer
See all Lysis Buffer reagents -
Tested applications
Suitable for: WB, ELISA, SDS-PAGE, IPmore details -
General notes
Abcam’s 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.
Preparation: Dilute to 1X in deionized water
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Form
Liquid -
Storage instructions
Shipped at Room Temperature. Store at Room Temperature. -
Storage buffer
pH: 7.50
Constituents: 0.22% Beta glycerophosphate, 0.18% Sodium orthovanadate, 5% Sodium deoxycholate, 0.38% EGTA, 1% Sodium lauryl sulfate, 6.1% Tris, 0.29% EDTA, 8.8% Sodium chloride, 1.12% Sodium pyrophosphate decahydrate, 10% Nonylphenol, ethoxylated -
Concentration information loading... -
Research areas
Associated products
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Related Buffer
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Suggested working concentration: 1X
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| ELISA |
Use at an assay dependent concentration. Suggested working concentration: 1X
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| SDS-PAGE |
Use at an assay dependent concentration. Suggested working concentration: 1X
|
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| IP |
Use at an assay dependent concentration. Suggested working concentration: 1X
|
| Notes |
|---|
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WB
Use at an assay dependent concentration. Suggested working concentration: 1X |
|
ELISA
Use at an assay dependent concentration. Suggested working concentration: 1X |
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SDS-PAGE
Use at an assay dependent concentration. Suggested working concentration: 1X |
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IP
Use at an assay dependent concentration. Suggested working concentration: 1X |
Images
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SDS-PAGE - 10X RIPA Buffer (ab156034)HeLa cell extraction using ab156034.
2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156034. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes: the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.
BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.1mg of protein was recovered and 82% was in the soluble cleared cell lysate.Lane 1: MW marker
Lane 2: Cleared lysateLane 3: Non-soluble
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (67)
ab156034 has been referenced in 67 publications.
- Jansens RJJ et al. Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex. Cell Rep 40:111107 (2022). WB, IP, ICC ; Pig . PubMed: 35858564
- Xie L et al. The HIF-1a/p53/miRNA-34a/Klotho axis in retinal pigment epithelial cells promotes subretinal fibrosis and exacerbates choroidal neovascularization. J Cell Mol Med 25:1700-1711 (2021). PubMed: 33438362
- Foray C et al. Imaging temozolomide-induced changes in the myeloid glioma microenvironment. Theranostics 11:2020-2033 (2021). PubMed: 33500706
- Shati AA & El-Kott AF Acylated ghrelin protects against doxorubicin-induced nephropathy by activating silent information regulator 1. Basic Clin Pharmacol Toxicol 128:805-821 (2021). PubMed: 33547742
- Chen Q et al. Rho-kinase inhibitor hydroxyfasudil protects against HIV-1 Tat-induced dysfunction of tight junction and neprilysin/Aß transfer receptor expression in mouse brain microvessels. Mol Cell Biochem 476:2159-2170 (2021). PubMed: 33548010