20x TBS-T with Tween 20 (ab64204)
Overview
-
Product name
20x TBS-T with Tween 20
See all TBS reagents -
Tested applications
Suitable for: IHC-Pmore details -
General notes
Tris Buffered Saline (TBS) wash buffer with Tween 20 for use during immunolabeling techniques such as IHC.
The salts in 20x solution are at a very high concentration. It has been found that at temperatures below 44 degrees F (6 degrees C), the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution.
Properties
-
Form
Liquid -
Storage instructions
Store at room temperature. -
Storage buffer
pH: 7.2
Constituents: 99% Tris buffered saline (x20), 1% Tween
20x Concentrated -
Concentration information loading... -
Research areas
-
Relevance
TBS is a solution of Tris Buffered Saline for use with immunolabeling techniques as a wash buffer. Tween 20 is sometimes added to promote more effective washings, resulting in decreased non-specific background staining. Tween 20 is also known to promote reagent spreading in automated staining methodologies. -
Alternative names
- Tris buffered saline
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent dilution. |
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 20x TBS-T with Tween 20 (ab64204)ab64204 20X TBS with Tween 20 used along with an anti-CD20 antibody on Human Tonsil
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (1)
ab64204 has been referenced in 1 publication.
- Jiang Y et al. MAP30 promotes apoptosis of U251 and U87 cells by suppressing the LGR5 and Wnt/ß-catenin signaling pathway, and enhancing Smac expression. Oncol Lett 15:5833-5840 (2018). PubMed: 29556310
