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    products/reagents/antibody-diluent-ab64211.pdf

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Antibody Diluent (ab64211)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (17)

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Overview

  • Product name

    Antibody Diluent
    See all Antibody Diluent reagents
  • Tested applications

    Suitable for: IHC-Pmore details
  • General notes

    Antibody Diluent ab64211 is used to dilute primary antibodies to their working concentrations for use in IHC. It can also be used for convenient storage of diluted primary antibodies.

    The antibody diluent is formulated to reduce background and non-specific binding of the primary antibody. In most cases, antibodies can be stored in the diluent, at their working concentrations for IHC, for up to 24 months at 2-8°C.

    The diluent is based on a Tris buffer, and contains a stabilizing carrier protein, a wetting agent to reduce surface tension, an anti-microbial agent, and a green dye. It should not be used with HRP-conjugated primary antibodies.


    IHC protocol suitable for use with Antibody Diluent ab64211:
    For fluorescent IHC, skip step 3, incubate with fluorescent dye conjugated secondary at step 6, skip rest of steps, and mount with anti-fade mounting medium.

    1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.

    2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.

    3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.

    4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.

    5. Apply primary antibody in Antibody Diluent ab64211 and incubate.

    6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.

    7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.

    8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC substrate and incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.

    9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.

    10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.


    Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C.
  • Storage buffer

    pH: 7
    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • IHC Tools/ Reagents
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Buffer
    • Diluent

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent dilution.
Notes
IHC-P
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (17)

Publishing research using ab64211? Please let us know so that we can cite the reference in this datasheet.

ab64211 has been referenced in 17 publications.

  • Navickaite I  et al. Anatomical evidence of non-parasympathetic cardiac nitrergic nerve fibres in rat. J Anat 238:20-35 (2021). PubMed: 32790077
  • Yu TY  et al. Platelet-Rich Plasma Releasate Promotes Early Healing in Tendon After Acute Injury. Orthop J Sports Med 9:2325967121990377 (2021). PubMed: 33959667
  • Vélez EJ & Unniappan S Nesfatin-1 and nesfatin-1-like peptide suppress growth hormone synthesis via the AC/PKA/CREB pathway in mammalian somatotrophs. Sci Rep 10:16686 (2020). PubMed: 33028951
  • Rapacz-Leonard A  et al. The oxytocin-prostaglandins pathways in the horse (Equus caballus) placenta during pregnancy, physiological parturition, and parturition with fetal membrane retention. Sci Rep 10:2089 (2020). PubMed: 32034259
  • Wrobel LJ  et al. Effects of Beta-Blockers on Melanoma Microenvironment and Disease Survival in Human. Cancers (Basel) 12:N/A (2020). PubMed: 32353988
View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

The high affinity of this solvent to slides saves us a lot of antibody usage.

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
We applied Antibody Diluent (ab64244) on Ki67 IHC staining on paraffin-embedded rat bladder tissue. The high affinity of this solvent to slides saves us a lot of antibody usage. It really worked well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 27 2022

Question

Dear Sirs.

I have a question for you.

Can you please inform me on the average expiry dates of these products

1. anti CD38 ab (ab125038)

2. anti ZAP-70 ab, prediluted (ab31009)

3. anti CXCR4 ab (ab2074)

4. anti CD49d antitelo - anti integrin alpha4 ab (ab78964)

5. ab diluent (ab64211)

6. EXPOSE Mouse and Rabbt Specific HRP/AEC Detection IHC Kit(ab94706)

Also, is it available that these ab’s and reagents are delivered in smaller packages?

Very best regards,

Read More

Abcam community

Verified customer

Asked on Feb 24 2012

Answer

Thank you for your enquiry.

We do not provide expiry dates as such. For antibodies, we recommend they can be stored up to 1 year after delivery under the storage conditions stated on the datasheet.

For kits, we recommend 6months after delivery.

I am currently confirming regarding the antibody diluent (ab64211) and will let you know as soon as possible.

Please note our guarantee for all products is for 6 months after purchase.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

Answered on Feb 24 2012

Question

Dear Technical Team,

Hope this e-mail finds you well!

Kindly advise the exact concentration of Antibody Diluent (ab64211) as desired by the customer, since on its datasheetthe concentration is mentioned as 125ml at mg/ml.

Looking forward towards your valued response on it.

With Best Regards,

Read More

Abcam community

Verified customer

Asked on Jan 19 2012

Answer

Thank your or your inquiry.

Unfortunately, this is a mistake in the datasheet that is IT related. This product has no concentration per se.

Antibody diluent is prepared for use in immunochemical procedures and antibodies can be diluted directly in this reagent.

The formulation of this diluent is the following:

1% BSA, PBS, pH 7.4 with 0.05% Sodium Azide as a preservative.

Thank you for making us aware of this mistake. Our IT department will take care of this as soon as possible.

Please do not hesitate to contact me again if you have any further questions.

Read More

Abcam Scientific Support

Answered on Jan 19 2012

Question

Dear abcam I’m currently looking for a stable antibody diluent for our automatic immunostainer. We need a diluent to be stable overnight in room temperature since we are planning to load up the stainer at the end of the work day for a delay schedule staining. How long does your antibody diluent (ab64211) can be kept in room temperature and be able to keep stable? Thanks

Read More

Abcam community

Verified customer

Asked on Dec 01 2011

Answer

Thank you for contacting us. We do not have any experimental data regarding the stability of our Antibody Diluent at room temperature, as our storage recommendation for this product is at 4C. In-house we have prepared our working antibody solutions in the morning and they are left at room temperature for 4-6 hrs until application to the slide and no stability issues have been seen with those particular antibodies; however I cannot guarantee that it would be stable overnight for your application. I am sorry I couldn’t be more helpful. Please do not hesitate to contact us if you need further advice or information.

Read More

Abcam Scientific Support

Answered on Dec 01 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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