Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
Overview
-
Product name
Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) -
Tested applications
Suitable for: IHC-Pmore details -
General notes
Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require Tris-EDTA buffer (pH 9.0) pretreatment.
This product contains detergent for emulsification of the paraffin.
1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.
Protocol:
- Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
- Wash sections with hot tap water for 1 minute.
- Wash sections in buffer for 2x3 minutes.
- Continue with IHC protocol
Other kits and reagents for IHC
Other antigen retrieval buffers include: Citrate buffer pH 6.0 ab93678, EDTA buffer pH 8.0 ab93680, Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Properties
-
Form
Liquid -
Storage instructions
Store at room temperature. -
Storage buffer
pH: 8.7
Constituents: 1.5% 2-butoxyethanol, 5% Sodium EDTA, 5% Tris
Buffer 100x concentrated with detergent. -
Concentration information loading... -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent dilution. |
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)Image from Maniati et al., Cell Rep.;30(2):525-540.e7; doi: 10.1016/j.celrep.2019.12.034. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Immunohistochemical for RUNX2 on murine omental metastasis Antigen retrieval: Tris-EDTA, pH;9 (ab93684), Blocking: Normal Goat Serum, Primary: Rabbit monoclonal anti-Runx2 [EPR14334] (ab192256), dilution 1:1000, Secondary: HRP anti-rabbit
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)ab93684 Antigen Retrieval Buffer 100X Tris-EDTA Buffer, pH 9 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling LINE-1 ORF1p with ab216324 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on spermatogonia of mouse testis (PMID: 24607009) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (22)
ab93684 has been referenced in 22 publications.
- Zhou LM et al. Nucleophosmin 1 overexpression correlates with 18F-FDG PET/CT metabolic parameters and improves diagnostic accuracy in patients with lung adenocarcinoma. Eur J Nucl Med Mol Imaging 48:904-912 (2021). PubMed: 32856112
- Lee H et al. The utility of high-mobility group A2 overexpression for predicting the prognosis of gastric cancer patients and its contribution to poor prognosis via chemoresistance and the propensity for the occurrence of carcinomatosis peritonei. Surgery 169:1213-1220 (2021). PubMed: 33376002
- Margaroli C et al. Spatial mapping of SARS-CoV-2 and H1N1 lung injury identifies differential transcriptional signatures. Cell Rep Med 2:100242 (2021). PubMed: 33778787
- Hortells L et al. Periostin-expressing Schwann cells and endoneurial cardiac fibroblasts contribute to sympathetic nerve fasciculation after birth. J Mol Cell Cardiol 154:124-136 (2021). PubMed: 33582160
- Santoro R et al. Modulating TAK1 Expression Inhibits YAP and TAZ Oncogenic Functions in Pancreatic Cancer. Mol Cancer Ther 19:247-257 (2020). PubMed: 31562256