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  1. Link

    products/reagents/aqueous-mounting-medium-ab128982.pdf

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Aqueous Mounting Medium (ab128982)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (7)

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Overview

  • Product name

    Aqueous Mounting Medium
    See all mounting media reagents
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-Pmore details
  • General notes

    Aqueous mounting medium for use in fluorescence microscopy. Enhances FITC intensity and reduces fluorescence fading. Ready-to-use. 

    Reagent: 6 ml

    Other products for IHC

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

    Procedure:

    FOR USE IN FLUORESCENCE MICROSCOPY

    After completion of immunostaining, remove slide from water, apply sufficient drops of Aqueous Mounting Medium upon section and mount coverglass. Coverslip should be immobilized within 30 minutes at room temperature. To avoid air bubbles within mounting medium, DO NOT SHAKE VIAL. If air bubbles are evident during coverslipping, carefully trim the vial tip with a sharp blade to create a larger opening and improve media flow. These directions are recommendations; however, they may be altered according to procedures established in-house.

    IHC Protocol Summary

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C. Do not freeze.
  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • IHC Tools/ Reagents
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Mounting Medium

Associated products

  • Related Products

    • Mounting Medium for IHC - Aqueous (ab64230)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (7)

Publishing research using ab128982? Please let us know so that we can cite the reference in this datasheet.

ab128982 has been referenced in 7 publications.

  • Franco-Barraza J  et al. Engineering clinically-relevant human fibroblastic cell-derived extracellular matrices. Methods Cell Biol 156:109-160 (2020). PubMed: 32222216
  • Kwok SS  et al. Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts. J Clin Med 9:N/A (2020). PubMed: 33171906
  • Wei K  et al. Defining the Vulnerability Window of Anesthesia-Induced Neuroapoptosis in Developing Dentate Gyrus Granule Cells - A Transgenic Approach Utilizing POMC-EGFP Mice. Neuroscience 415:59-69 (2019). PubMed: 31301366
  • Sun X  et al. (-)-Epigallocatechin-3-gallate inhibits bladder cancer stem cells via suppression of sonic hedgehog pathway. Oncol Rep 42:425-435 (2019). PubMed: 31180522
  • Shaalan A & Proctor G Salivary glands require Aurora Kinase B for regeneration after transient innate immune-mediated injury. Sci Rep 9:11339 (2019). PubMed: 31383943
  • Shaimerdenova M  et al. The effects of antiviral treatment on breast cancer cell line. Infect Agent Cancer 12:18 (2017). PubMed: 28344640
  • Dong C  et al. Ketamine Affects the Neurogenesis of Rat Fetal Neural Stem Progenitor Cells via the PI3K/Akt-p27 Signaling Pathway. Birth Defects Res B Dev Reprod Toxicol 101:355-63 (2014). PubMed: 25231110

Customer reviews and Q&As

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1-2 of 2 Abreviews

Aqueous Mounting Medium

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
The medium is very good to cover immune stainings. It is easy to dose and use. The preparations are clear and easy to microscopy.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 29 2019

nice item, fast shipping

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
This mounting medium has very good quality. It does not cause bubbles if you are very careful and only use a small drop. My slides are still good several months after using the mounting medium.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 23 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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