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    products/reagents/cal-520-am-ab171868.pdf

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Signal Transduction Signaling Pathway Calcium Signaling Other
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Cal-520, AM (ab171868)

  • Datasheet
Reviews (1) Submit a question References (6)

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Immunocytochemistry - Cal-520, AM (ab171868)

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    Overview

    • Product name

      Cal-520, AM
    • General notes

      ab171868 provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520 AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). Cells expressing a GPCR or calcium channel of interest that signals through calcium can be preloaded with Cal-520 AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Cal 520 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells. Its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which significantly increase the fluorescence of Cal-520. The characteristics of its long wavelength, high sensitivity, and >100 times fluorescence enhancement, make Cal-520 AM an ideal indicator for the measurement of cellular calcium. The high S/N ratio and better intracellular retention make the Cal-520 calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists.

      Molecular Weight (MW) 1102.95 Da.

    Properties

    • Form

      Solid
    • Storage instructions

      Store at -20°C. Store In the Dark.
    • Storage buffer

      Constituent: 100% Cal-520®, AM
    • Concentration information loading...
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Calcium Signaling
      • Other
      • Signal Transduction
      • Metabolism
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      • Calcium assay kits
    • Relevance

      Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.
    • Alternative names

      • Ca++
      • Ca2+

    Images

    • Immunocytochemistry - Cal-520, AM (ab171868)
      Immunocytochemistry - Cal-520, AM (ab171868)
      CHO-M1 cells were seeded overnight at 40,000 cells per 100 μL per well in a 96-well black wall/clear bottom plate. 100 μl of 4 μM Cal 520 AM in HHBS were added into the wells, and the cells were incubated at 37 °C for 2 hour.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (6)

    Publishing research using ab171868? Please let us know so that we can cite the reference in this datasheet.

    ab171868 has been referenced in 6 publications.

    • Bergmann GA & Bicker G Cholinergic calcium responses in cultured antennal lobe neurons of the migratory locust. Sci Rep 11:10018 (2021). PubMed: 33976252
    • Velasco-Estevez M  et al. Piezo1 regulates calcium oscillations and cytokine release from astrocytes. Glia 68:145-160 (2020). PubMed: 31433095
    • Lowin T  et al. Cannabidiol (CBD): a killer for inflammatory rheumatoid arthritis synovial fibroblasts. Cell Death Dis 11:714 (2020). PubMed: 32873774
    • Dave A  et al. Mitochondrial Electron Transport Chain Complex Dysfunction in MeCP2 Knock-Down Astrocytes: Protective Effects of Quercetin Hydrate. J Mol Neurosci 67:16-27 (2019). PubMed: 30519865
    • Bai L  et al. A Conserved Circadian Function for the Neurofibromatosis 1 Gene. Cell Rep 22:3416-3426 (2018). PubMed: 29590612
    • Wilson C  et al. Acetylcholine released by endothelial cells facilitates flow-mediated dilatation. J Physiol 594:7267-7307 (2016). PubMed: 27730645

    Customer reviews and Q&As

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    Very good signal

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Cal-520 used for calcium imaging on primary astrocyte culture.
    Used following the protocol provided and imaged under a Leica Sp6.

    Figure included is an example of image when astrocytes are stimulated with ATP (50uM).
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Maria Velasco

    Verified customer

    Submitted Jun 05 2018

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