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    products/reagents/cytrak-orange-50l-5-mm-ab109203.pdf

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CyTRAK Orange™ 50µl (5 mM) (ab109203)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - CyTRAK Orange™ 50µl (5 mM) (ab109203)

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    Overview

    • Product name

      CyTRAK Orange™ 50µl (5 mM)
    • Tested applications

      Suitable for: FM, Flow Cyt, ICC/IFmore details
    • General notes

      CyTRAK Orange™ is a novel orange fluorescent dye [related to DRAQ5™ (ab108410)] that stains both nucleus and cytoplasm. It is water soluble and membrane permeant which can be used in live and fixed cells in combination with other common fluorophores, such as GFP-fusions, green-labelled antibodies (FITC or DyLight® 488) and far-red dyes. CyTRAK Orange™ preferentially stains the nucleus, but also defines the cytoplasmic area in both live and fixed preparations. CyTRAK Orange™ does the work of two fluorophores! Key features of CyTRAK Orange™ include:

      • Rapid staining of dsDNA/nuclei of LIVE or fixed cells
      • Differential intensity of DNA binding and Cytoplasmic labelling - One fluorophore doing the work of two
      • Low photobleaching effect
      • Ideal for use with GFP, FITC & DyLight® 488 and 649 labels
      • No lyse / no wash protocols
      • It is membrane-permeant

      Excitation

      • 457, 488 & 549 (Exmax 515nm)
      • similar profile to Propidium Iodide
      • co-excited with FITC/Cy2/eGFP and PE-Cy7
      • NOT excited by UV or red lasers (e.g. 633 & 647 nm)

      Emission (instrument dependent):

      • Emmax 615 nm
      • No significant spectral overlap with co-excited green fluors (eGFP, FITC, Cy2)
      • Visualize GFP-tagged temporospatial changes in live cells in a single acquisition from a 488 nm excitation

    Properties

    • Form

      Liquid
    • Storage instructions

      Store at +4°C. Do Not Freeze. Store In the Dark.
    • Concentration information loading...
    • Research areas

      • Kits/ Lysates/ Other
      • Tools and Reagents
      • IHC Tools/ Reagents
      • Kits/ Lysates/ Other
      • Tools and Reagents
      • Fluorescent dyes and reagents
      • Kits/ Lysates/ Other
      • Kits
      • Cell Staining Kits
      • Nuclear

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab109203 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    FM
    Use at an assay dependent concentration.
    Flow Cyt
    1/300 - 1/1000.

    For Nucleated Cell Gate = 5µM; For Discrimination of Arrested/ Senescent Cells = 15µM

    ICC/IF
    1/1000. For microscopy counterstain = 5µM
    It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet.
    Notes
    FM
    Use at an assay dependent concentration.
    Flow Cyt
    1/300 - 1/1000.

    For Nucleated Cell Gate = 5µM; For Discrimination of Arrested/ Senescent Cells = 15µM

    ICC/IF
    1/1000. For microscopy counterstain = 5µM
    It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet.

    Images

    • Immunocytochemistry/ Immunofluorescence - CyTRAK Orange™ 50µl (5 mM) (ab109203)
      Immunocytochemistry/ Immunofluorescence - CyTRAK Orange™ 50µl (5 mM) (ab109203)
      Live cells expressing eGFP/CyclinB1 were labelled with CyTRAK Orange™ (end concentration 20µM) for 20 minutes at 37 C, no wash. Image was captured using confocal microscopy at 488nm simultaneously: i) CyTRAK Orange™ channel - 598/40nm; ii) eGFP/CyclinB1 channel - 522/35nm

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab109203? Please let us know so that we can cite the reference in this datasheet.

    ab109203 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    Bonjour,
    A ce jour, je n’ai pas reçu le devis n°****.
    Pouvez-vous me le re-expédier.
    Merci.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 21 2012

    Answer

    Je vous ai envoyé de nouveau le devis ****.

    Par précaution je vous envoie en pièce jointe le pdf de ce même devis.

    J'espère que vous ferez bonne réception de ce document. Vérifiez votre dossier "courrier indésirable", le devis s'y est peut-être glissé.

    N'hésitez pas à me contacter de nouveau si vous avez d'autres questions.

    Read More

    Abcam Scientific Support

    Answered on Sep 21 2012

    Question

    Bonjour,
    Merci pour votre mail.
    Pouvez-vous ajouter au devis en plus du DRAQ5 et du CyTRAQ Orange, la référence suivante:
    CytoPainter Mitochondrial Staining Kits réf: ab112144

    Read More

    Abcam community

    Verified customer

    Asked on Sep 20 2012

    Answer

    Merci pour votre réponse.

    Un devis vous a été envoyé dans un autre email. La référence du devis est *****.

    N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Abcam Scientific Support

    Answered on Sep 20 2012

    Question

    fluorescent cytoplasmic markers

    Read More

    Abcam community

    Verified customer

    Asked on Sep 20 2012

    Answer

    Merci de nous avoir contactés.

    Nous avons récemment ajouté à notre catalogue une gamme de produits de marquage fluorescentappelés kits CytoPainter qui permettent de marquer les organites tels que les mitochondries, lysosomes et filaments d'actine.
    Le lien vers la liste de ces kits est : https://www.abcam.com/CytoPainter.

    Merci de m'indiquer si vous désirez ajouter un de ces produits au devis que vous m'avez demandé pour le DRAQ5 et CyTRAQ Orange.

    Read More

    Abcam Scientific Support

    Answered on Sep 20 2012

    Question

    I have just purchased CyTRAK Orange from your company and I have some small questions about this: 1. CyTRAK Orange can be used for live cell staining. So what kind of effect does CyTRAK have on cell behaviors? 2. I want to take time lapse movie of live cell for 96 h. Is it possible to used CyTrak for this experiment? Thank you for your time!

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2011

    Answer

    Thank you for your enquiry. This CyTRAK Orange (ab109203) is a novel orange fluorescent dye [related to DRAQ5™ (ab108410)] that stains both nucleus and cytoplasm. Please rest assured that it too can be used in live cell imaging. However, we have not tested if there are any effect for experiments up to 96 hr but do not foresee any complications. I would suggest that you also run a positive control (without CyTRAK) to confirm any results you get. I hope I have answered your questions. If there is anything else that I can help you with please do not hesitate to contact me. Have a nice day!

    Read More

    Abcam Scientific Support

    Answered on Aug 08 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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