DAB Enhancer (ab675)
Overview
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Product name
DAB Enhancer -
General notes
To use as DAB signal enhancer in conjunction with peroxidase based immunostaining systems.
i) Add one drop (50µL) of DAB enhancer in 1mL of DAB incubation buffer.
ii) Add DAB chromogen and Peroxidase Block (3% Hydrogen Peroxide) to the above buffer.
iii) Incubate tissue sections with the incubation medium.
DAB is a widely used chromogen for immunoperoxidase staining and immunoblotting. It has been well accepted amongst pathologists because of its superior performance as compared to amino ethylcarbazole (AEC). DAB gives cleaner background as opposed to AEC. Specimens stained in DAB can be dehydrated in ethanol and cleared in xylene and can be mounted for permanent record keeping. Strength of signal generated during the immunostaining is the key to a good staining. This is a stable liquid solution which increases the staining intensity of the tissue specimens stained with DAB several fold and hence increases the efficiency of detection systems.
Properties
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Form
Liquid -
Storage instructions
Shipped at Room Temperature. Store at Room Temperature. -
Concentration information loading... -
Reagent notes
DAB is a widely used chromogen for immunoperoxidase staining and immunoblotting. It has been well accepted amongst pathologists because of its superior performance as compared to amino ethylcarbazole (AEC). DAB gives cleaner background as opposed to AEC. Specimens stained in DAB can be dehydrated in ethanol and cleared in xylene and can be mounted for permanent record keeping. Strength of signal generated during the immunostaining is the key to a good staining. This is a stable liquid solution which increases the staining intensity of the tissue specimens stained with DAB several fold and hence increases the efficiency of detection systems. -
Research areas
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab675 has been referenced in 2 publications.
- Zhang Z et al. LncRNA BC032020 suppresses the survival of human pancreatic ductal adenocarcinoma cells by targeting ZNF451. Int J Oncol 52:1224-1234 (2018). PubMed: 29532883
- Fernandez S et al. Nasal immunity to staphylococcal toxic shock is controlled by the nasopharynx-associated lymphoid tissue. Clin Vaccine Immunol 18:667-75 (2011). PubMed: 21325486