DRAQ7™ (ab109202)
Overview
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Product name
DRAQ7™ -
Tested applications
Suitable for: FM, Flow Cyt, ICC/IFmore details -
General notes
DRAQ7™ is a far-red fluorescent dye that only stains the nuclei in dead and permeabilized cells and can be used in combination with common labels such as GFP or FITC.
DRAQ7 is the ideal tool to study dead or membrane-compromised cells because it does not enter intact, live cells. It is an ideal replacement for propidium iodide and 7-AAD, as is not excited by UV light and has no emission overlap with PE / PE homologues.
Key features of DRAQ7 include:
- Rapid staining of dsDNA/ nuclei of dead or permeabilized cells
- Low photobleaching
- It can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc ...
- Non-toxic in long-term culture
- Can be combined with "live" dyes
- No compensation needed with common FITC/GFP + PE combinations in flow cytometry
- No wash or RNase treatment needed.
SPECTRAL PROPERTIES
Excitation:
- 633 and 647 nm line optimal (Exmax 599 / 644 nm)
- 488, 514 and 568 nm lines, sub-optimal (only by flow cytometry)
Emission (instrument dependent):
- 665 nm to infra-red max 678 nm / 697 nm intercalated with dsDNA)
- Minimal overlap with vis range e.g. GFP and FITC
- Em. filters may include 695L, 715LP or 780 LP
Multi-wavelength imaging with UV / vis fluorochromes
- No fluorescence enhancement upon DNA binding
- Low photo-bleaching effect
- Compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluoroscence microscopes
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. Do Not Freeze. Store In the Dark. -
Concentration information loading... -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| FM |
Use at an assay dependent concentration.
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| Flow Cyt |
1/100. (final concentration = 3µM)
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| ICC/IF |
1/100. (final concentration = 3µM)
It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet. |
| Notes |
|---|
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FM
Use at an assay dependent concentration. |
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Flow Cyt
1/100. (final concentration = 3µM) |
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ICC/IF
1/100. (final concentration = 3µM) It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet. |
Images
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Flow Cytometry - DRAQ7™ (ab109202)Jurkat cells exposed to 1µM staurosporine for 24 hours show DRAQ7™ staining (top half of the plot). These cells have compromised membranes that allow entry of DRAQ7™ in the cells.
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Immunohistochemistry (Frozen sections) - DRAQ7™ (ab109202)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolSample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 μm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody: Goat anti-rabbit IgG - H&L (AMCA) (ab123435)
Nuclei were counterstained stained with DRAQ7™ (ab109202) -
Immunohistochemistry (Frozen sections) - DRAQ7™ (ab109202)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolSample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4°C overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 μm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050), 1:200
F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1:1000
Nuclei were counterstained stained with DRAQ7™, 1:1000
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab109202 has been referenced in 29 publications.
- Mehta C et al. RNA-regulatory exosome complex confers cellular survival to promote erythropoiesis. Nucleic Acids Res N/A:N/A (2021). PubMed: 34059908
- Boon R et al. Amino acid levels determine metabolism and CYP450 function of hepatocytes and hepatoma cell lines. Nat Commun 11:1393 (2020). PubMed: 32170132
- Giovinazzo JA et al. Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity. Elife 9:N/A (2020). PubMed: 32427098
- Bradford MK & Elkins KL Immune lymphocytes halt replication of Francisella tularensis LVS within the cytoplasm of infected macrophages. Sci Rep 10:12023 (2020). PubMed: 32694562
- Malaquin N et al. DNA Damage- But Not Enzalutamide-Induced Senescence in Prostate Cancer Promotes Senolytic Bcl-xL Inhibitor Sensitivity. Cells 9:N/A (2020). PubMed: 32630281