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    products/reagents/dykddddk-tag-affinity-beads-binds-to-flag-tag-sequence-ab270704.pdf

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Anti-DYKDDDDK tag Affinity Beads (Binds to FLAG® tag sequence) (ab270704)

  • Datasheet
  • SDS
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Overview

  • Product name

    Anti-DYKDDDDK tag Affinity Beads (Binds to FLAG® tag sequence)
  • General notes

    Anti-DYKDDDDK tag Affinity Beads (Binds to FLAG® tag sequence) (ab270704) is an affinity chromatography medium designed for easy, one-step purification or immunoprecipitation of DYKDDDDK (flag) at N-terminal, C-terminal and internal locations of a fusion protein. Anti-DYKDDDDK Affinity Beads are coupled with an anti-DYKDDDDK monoclonal antibody by a technique which generates a stable linkage between the antibody and the agarose beads.

    The characteristics of the resin:

    Item

    Description

    Matrix

    4% agarose beads

    Ligand

    Anti-DYKDDDDK Antibody

    Binding Capacity

    >1mg DYKDDDDK fusion protein/mL medium

    Particle size (μm)

    45–165

    Max Pressure

    0.1 MPa, 1 bar

    Storage Buffer

    PBS buffer with 0.02% sodium azide

    Storage Temperature

    4ºC


    For the purification of DYKDDDDK-tagged fusion proteins, Anti-DYKDDDDK tag Affinity Beads can be used in column chromatography, batch format and immunoprecipitation.

    For large volumes of sample, column chromatography or batch format are recommended to quickly capture the target protein from a large volume of extract.

    When a small amount of sample is being purified, immunoprecipitation procedure is recommended.

    Notes

    This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra® Anti-DYKDDDDK Affinity Beads. Expedeon product codes AF0001 (1 mL Medium), AF0005 (5 mL Medium), AF0010 (10mL Medium), AF0025 (25 mL Medium), and AF0050 (50 mL Medium) are the same as the current sizes: 1 mL, 5 mL, 10 mL, 25 mL, and 50 mL, respectively.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at Room Temperature. Store at +4°C. Do Not Freeze. Please see notes section.
  • Storage buffer

    pH: 6.00
    Preservative: 0.02% Sodium azide
    Constituent: 49.98% PBS
  • Concentration information loading...

Protocols

  • Protocol Booklet

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Datasheets and documents

  • SDS download

  • Datasheet download

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References (0)

Publishing research using ab270704? Please let us know so that we can cite the reference in this datasheet.

ab270704 has not yet been referenced specifically in any publications.

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