Hematoxylin Solution (Mayer's, Modified) (ab220365)
Overview
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Product name
Hematoxylin Solution (Mayer's, Modified) -
Tested applications
Suitable for: IHCmore details -
General notes
Hematoxylin solution ab220365 is a high quality, ready-to-use solution of a commonly used formulation called Modified Mayer's hematoxylin (Lille's modification).
This hematoxylin solution is also available in H&E staining kit ab245880.
Hematoxylin is a nuclear stain that is used as a counterstain for immunohistochemistry (IHC). Together with eosin, it forms the Hematoxylin and Eosin stain (H&E stain), one of the most commonly used stains in histology.
Other products for IHC
Find kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Properties
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Form
Liquid -
Storage instructions
Store at room temperature. -
Storage buffer
pH: 2.00
Constituents: 30% Glycerol (glycerin, glycerine), 5% Aluminium sulfate, 2% Acetic acid, 1% Haematoxylin -
Concentration information loading...
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC |
Use at an assay dependent concentration.
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Notes |
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IHC
Use at an assay dependent concentration. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hematoxylin Solution (Mayer's, Modified) (ab220365)
Immunohistochemistry Staining with CD34 on Human tonsil. Counterstained with Hematoxylin Solution (Mayer's, Modified) (ab220365) for 30 seconds followed by bluing with Bluing Reagent.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hematoxylin Solution (Mayer's, Modified) (ab220365)
H&E Stain Kit (Hematoxylin and Eosin) staining formalin-fixed-paraffin embedded tissue section.
Hematoxylin Solution (Mayer's, Modified) ab220365 is used as a component of H&E Staining Kit (Hematoxylin and Eosin) ab245880.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hematoxylin Solution (Mayer's, Modified) (ab220365)
Pig Lung stained with Hematoxylin Solution (Mayer's,Modified) (ab220365) followed by bluing with Bluing Reagent.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab220365 has been referenced in 14 publications.
- Gao R et al. Macrophage-derived netrin-1 drives adrenergic nerve-associated lung fibrosis. J Clin Invest 131:N/A (2021). PubMed: 33393489
- Wang J et al. SCARA5 suppresses the proliferation and migration, and promotes the apoptosis of human retinoblastoma cells by inhibiting the PI3K/AKT pathway. Mol Med Rep 23:N/A (2021). PubMed: 33495818
- Zuo S et al. Enhanced antitumor efficacy of a novel oncolytic vaccinia virus encoding a fully monoclonal antibody against T-cell immunoglobulin and ITIM domain (TIGIT). EBioMedicine 64:103240 (2021). PubMed: 33581644
- Wu M et al. ID1 overexpression promotes HCC progression by amplifying the AURKA/Myc signaling pathway. Int J Oncol 57:845-857 (2020). PubMed: 32705157
- Park SH et al. Water Extract of Lotus Leaf Alleviates Dexamethasone-Induced Muscle Atrophy via Regulating Protein Metabolism-Related Pathways in Mice. Molecules 25:N/A (2020). PubMed: 33050143