HRP Stability Conjugate Reagent (ab171537)
Overview
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Product name
HRP Stability Conjugate Reagent
See all ELISA buffer reagents -
Tested applications
Suitable for: ELISAmore details
Unsuitable for: IHC-Fr or IHC-P -
General notes
Abcam’s HRP Stability Conjugate Reagent (ab171537) was developed specifically for Horseradish Peroxidase (HRP) conjugates in solution. ab171537 is readily incorporated into virtually any assay production protocol. Conjugates can be added directly to ab171537 and are then ready for storage and use. It is also an excellent diluent, and often decreases background and increases signal for improved signal-to-noise ratios. ab171537 prevents the loss of catalytic activity while it maintains the conformation of the antibody or protein-antigen portion of the conjugate.
98% Proprietary component
Recommendations for Use of ab171537 HRP Stability Conjugate Reagent
- For optimal stability and performance, use ab171537 at 100% concentration
- Dilute your HRP conjugate to optimum working concentration in the HRP Stability Conjugate Reagent solution
- Use diluted conjugate working solution in your assay as normal
- Or, preferably, store away from direct light at room temperature and use as a stock solution for optimum assay-to-assay consistency
NOTE – When storing any conjugate/ HRP Stability Conjugate Reagent solution for an extended period of time: If a phosphate buffer is used for dilution we recommend a final concentration of no more than 5 mM buffer to avoid the possibility of initiating precipitation.
Properties
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Form
Liquid -
Storage instructions
Store at room temperature. -
Storage buffer
pH: 6.20
Preservative: 0.02% 5-Bromo-5-nitro-1,3-dioxane
Constituents: 0.02% 2-methyl-4-isothiazolin-3-one, 0.0024% StabilZyme® HRP Conjugate Stabilizer
Further information:
Complete pH range: 6.2 - 6.7
98% Proprietary component
Contains BSA -
Concentration information loading...
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Images
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HRP Stability Conjugate Reagent (ab171537) efficacy was verified by real-time stability studies that included independent testing of the enzyme activity and the immunological activity by ELISA.
The studies were conducted using a monoclonal IgG-HRP conjugate. Results of these analyses indicate superior performance of ab171537 compared to a common HRP conjugate diluent. The monoclonal IgG-HRP conjugate retained over 90% of its activity when stored in HRP Stability Conjugate Reagent for 24 months at room temperature.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab171537 has not yet been referenced specifically in any publications.