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    products/reagents/hydrogen-peroxide-blocking-reagent-ab64218.pdf

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Hydrogen Peroxide Blocking Reagent (ab64218)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (19)

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Immunohistochemistry (Frozen sections) - Hydrogen Peroxide Blocking Reagent (ab64218)

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    Overview

    • Product name

      Hydrogen Peroxide Blocking Reagent
    • Tested applications

      Suitable for: IHC-P, IHC-Frmore details
    • General notes

      Hydrogen Peroxide Blocking Reagent ab64218 for use in IHC with detection based on HRP / peroxidase. 

      Note: The concentration of hydrogen peroxide in this product is 0.3%.


      IHC protocol suitable for use with Hydrogen Peroxide Blocking Reagent:
      For frozen sections, skip steps 1 and 2. 

      1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.

      2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.

      3. Add enough hydrogen peroxide blocking solution ab64218 to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.

      4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.

      5. Apply primary antibody in antibody diluent and incubate.

      6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.

      7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.

      8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC substrate and incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.

      9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.

      10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.


      Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

    Properties

    • Form

      Liquid
    • Storage instructions

      Store at +4°C.
    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: 0.3% Hydrogen peroxide
    • Concentration information loading...
    • Research areas

      • Kits/ Lysates/ Other
      • Tools and Reagents
      • IHC Tools/ Reagents
      • Kits/ Lysates/ Other
      • Kits
      • IHC tools
      • Block
      • Hydrogen Peroxide Block
    • Relevance

      Hydrogen Peroxide Blocking Reagent is intended for use in peroxidase-based immunohistochemical staining procedures on cell preparations, frozen tissue sections, and paraffin-embedded tissue sections.
    • Alternative names

      • Hydrogen peroxide block

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab64218 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Notes
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.

    Images

    • Immunohistochemistry (Frozen sections) - Hydrogen Peroxide Blocking Reagent (ab64218)
      Immunohistochemistry (Frozen sections) - Hydrogen Peroxide Blocking Reagent (ab64218)Selby M.J et al., PLoS One 11(9), Fig 2. doi: 10.1371/journal.pone.0161779. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block (ab64218) to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437. Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.

       

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (19)

    Publishing research using ab64218? Please let us know so that we can cite the reference in this datasheet.

    ab64218 has been referenced in 19 publications.

    • Cheng YH  et al. Extracorporeal Shock Wave Therapy Combined with Platelet-Rich Plasma during Preventive and Therapeutic Stages of Intrauterine Adhesion in a Rat Model. Biomedicines 10:N/A (2022). PubMed: 35203684
    • Martins M  et al. From Deer-to-Deer: SARS-CoV-2 is efficiently transmitted and presents broad tissue tropism and replication sites in white-tailed deer. PLoS Pathog 18:e1010197 (2022). PubMed: 35312736
    • Lebovitz C  et al. Loss of Parkinson's susceptibility gene LRRK2 promotes carcinogen-induced lung tumorigenesis. Sci Rep 11:2097 (2021). PubMed: 33483550
    • Carvallo FR  et al. Severe SARS-CoV-2 Infection in a Cat with Hypertrophic Cardiomyopathy. Viruses 13:N/A (2021). PubMed: 34452375
    • Kondo M  et al. Safety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment. NPJ Regen Med 6:65 (2021). PubMed: 34654830
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    really good working kit

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    We used this kit to reduce background staining produced by endogenous peroxidase .
    Its works good and is easy to use, because of the dropper bottle.
    We used the kit as a ready to use-kit and incubated the samples for 10minutes with the hydrogen peroxidase blocking reagent.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 12 2018

    IHC-P with Ki67 antibody using H2O2 blocking reagant

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    H2O2 blocking reagant was used prior to protein blocking, and primary antibody was diluted 1:100 and incubated at RT for 1 hr. As the image indicates, the peroxidase activity was significantly blocked and there was minimal background staining.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 16 2016

    Hydrogen peroxidase Blocking Solution

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Using HRP conjugated antibody may result in high, non-specific background staining. The non-specific background can be significantly reduced by pre-treatment of tissues with hydrogen peroxide prior to incubation with HRP conjugated antibody. Pictures show mouse gastrocnemius staining with no pre-treatment and pre-treatment with Hydrogen Peroxide Blocking solution from abcam. Results shows a significant improvement with an abolished background staining.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Francesco Elia Marino

    Verified customer

    Submitted Jul 23 2014

    Question


    I just wonder, what the difference between the 2 reagents would be?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 11 2013

    Answer


    ab64218 and ab94666 are exactly the same products but ab64218 is sold as 125 ml and ab94666 is sold as 60 ml.

    Read More

    Abcam Scientific Support

    Answered on Feb 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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