For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/reagents/mounting-medium-for-ihc-aqueous-ab64230.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Tools and Reagents IHC Tools/ Reagents
Share by email

Mounting Medium for IHC - Aqueous (ab64230)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (9)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mounting Medium (ab64230)

    You may also be interested in

    IHC
    Product image
    Hematoxylin Solution (Mayer's, Modified) (ab220365)
    Primary
    Product image
    PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)
    Secondary
    Goat Anti-Mouse Anti-Rabbit IgG/IgM H&L (HRP polymer) (ab2891)

    View more associated products

    Overview

    • Product name

      Mounting Medium for IHC - Aqueous
      See all mounting media reagents
    • Tested applications

      Suitable for: IHC-Pmore details
    • General notes

      Mounting Medium ab64230 is an aqueous mounting medium designed for use with chromogenic tissue and cell staining. It can be used with chromogens such as Fast-Red or AEC that require an aqueous mounting medium, or those that do not, such as DAB. When it is dry, it generates an even surface and can be used without a glass coverslip.

      Mounting media products

      For fluorescent cell and tissue staining, Abcam recommends aqueous, anti-fade Fluorescence Mounting Medium ab104135, Mounting Medium with DAPI ab104139, and Mounting Medium with PI ab104129. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI ab188804.

      For chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend this product, Mounting Medium ab64230, or organic Limonene Mounting Medium ab104141. 

       

      IHC protocol suitable for use with Mounting Medium ab64230:

      1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.

      2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.

      3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.

      4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.

      5. Apply primary antibody in antibody diluent and incubate.

      6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.

      7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.

      8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC Substrate and incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.

      9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.

      10. Rinse 7-8 times in tap water. Add Mounting Medium ab64230 to cover the section.


      Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

    Properties

    • Form

      Liquid
    • Storage instructions

      Store at room temperature.
    • Storage buffer

      Preservative: 0.1% Sodium azide
    • Concentration information loading...
    • Reagent notes

      ab64230 is formulated for coverslipping sections. It can be used with chromogens such as Fast-Red or AEC that require an aqueous mounting medium, or those that do not, such as DAB. When it is dry, it generates an even surface and can be used without a glass coverslip.
    • Research areas

      • Kits/ Lysates/ Other
      • Tools and Reagents
      • IHC Tools/ Reagents
      • Kits/ Lysates/ Other
      • Kits
      • IHC tools
      • Mounting Medium

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab64230 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use at an assay dependent dilution.
    Notes
    IHC-P
    Use at an assay dependent dilution.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mounting Medium (ab64230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mounting Medium (ab64230)
      ab64230 Mounting Medium used along with anti-CD20 antibody on Human Tonsil

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (9)

    Publishing research using ab64230? Please let us know so that we can cite the reference in this datasheet.

    ab64230 has been referenced in 9 publications.

    • Li M  et al. Long non-coding RNA AFAP1-AS1 facilitates the growth and invasiveness of oral squamous cell carcinoma by regulating the miR-145/HOXA1 axis. Oncol Rep 45:1094-1104 (2021). PubMed: 33650645
    • Tang T  et al. Pterostilbene reduces endothelial cell injury in vascular arterial walls by regulating the Nrf2-mediated AMPK/STAT3 pathway in an atherosclerosis rat model. Exp Ther Med 19:45-52 (2020). PubMed: 31853271
    • Tang Y  et al. Knockdown of eukaryotic translation initiation factor 5A2 enhances the therapeutic efficiency of doxorubicin in hepatocellular carcinoma cells by triggering lethal autophagy. Int J Oncol 57:1368-1380 (2020). PubMed: 33174013
    • Kole E  et al. Effects of melatonin on ovarian reserve in cigarette smoking: an experimental study. Arch Med Sci 16:1376-1386 (2020). PubMed: 33224337
    • Kim YS  et al. Novel Medicine for Endometriosis and Its Therapeutic Effect in a Mouse Model. Biomedicines 8:N/A (2020). PubMed: 33339236
    • Lu J  et al. Resveratrol alleviates inflammatory injury and enhances the apoptosis of fibroblast-like synoviocytes via mitochondrial dysfunction and ER stress in rats with adjuvant arthritis. Mol Med Rep 20:463-472 (2019). PubMed: 31180523
    • Liang Y  et al. Protective effect of extracorporeal membrane oxygenation on intestinal mucosal injury after cardiopulmonary resuscitation in pigs. Exp Ther Med 18:4347-4355 (2019). PubMed: 31777541
    • Chen YC  et al. Laser-emission imaging of nuclear biomarkers for high-contrast cancer screening and immunodiagnosis. Nat Biomed Eng 1:724-735 (2017). PubMed: 29204310
    • Nakamura F  et al. Documentation and localization of force-mediated filamin A domain perturbations in moving cells. Nat Commun 5:4656 (2014). PubMed: 25120197

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    xxxx;

    As I told you on the phone, I am planning on performing IHC on fresh PBMC samples from sheep.

    Cells will be isolated from whole blood using a density gradient and will be placed on glass slides using a cytocentrifuge. At this point, we CAN fix them if needed, but we don’t have to. What do you recommend?

    Our primary antibody is affinity purified rabbit polyclonal. We care considering using your EXPOSE Rabbit specific HRP-AES detection kit (ab94361) – do you think this is the best kit to use, or would you recommend a different one for our purposes? We are attempting to stain a transmembrane protein.

    We are planning to counterstain using hematoxylin: do you think we would get the best contrast with the AEC kit or would you recommend using DAB?

    Also, does abcam recommend purchasing hematoxylin from any particular vendor?

    If ab64230 the appropriate mounting medium to use with this kit?

    Thanks so much for your help. I look forward to your response!

    Read More

    Abcam community

    Verified customer

    Asked on Jun 20 2012

    Answer

    Thank you for replying with that information.

    As I mentioned over the phone, I have spoken to the source of these kits and we do not have any experimental data using either the ACE or DABdetection kits (ab64260, ab64261, ab80437 and ab94361)for IHC on fresh PBMC samples, asallof these kits have only been tested on formalin fixed paraffin embedded tissue samples.Wewould expect that as long as the primary antibody binds the target as expected that both kits could be suitable foryour samples but as we have not tested it ourselves we would not be able to guarentee this.



    There should be good contrast if using the AEC or DAB kit with hematoxylin as counterstain. We counterstain with hematoxylin in-house regardless ofwhether DAB or AEC is used. The sensitivity betweenAEC and DABis similar. I would recommend using ab94361 or ab80437 if you expect the PBMC samples to contain any endogenous biotin.I am sorry but I cannot recommend a particular supplier of hematoxylin.

    And to answer your final question, the mounting media ab64230 should be suitable for your purposes.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Jun 20 2012

    Question

    Dear Madam / Sir, A customer would like to buy a mounting medium to use IHC and IF. Which ABCAM mounting medium can be used with both of these applications? Thank you in advance.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 25 2012

    Answer

    Thank you for contacting us.

    Differences in the chemical compositions of the mounting medium make using one product for both IHC and IF difficult. The conditions required for excellent perioxidase preservation are not neccessarily good for the anti- photobleaching requirements of IF. As such I cannot recommend a single medium for you customer but suggest they by mounting mediums specific to their needs.

    Our standard mointing medium (ab64230;https://www.abcam.com/Mounting-Medium-ab64230.html) is appropriate for IHC.



    For IF I recommend BrightMount/Plus (ab103748;https://www.abcam.com/BrightMount-Plus-25ml-Aqueous-Mounting-Medium-Anti-fading-for-Fluorescent-Staining-ab103748.html)


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 25 2012

    Question

    Dear Technical Team, Hope this e-mail finds you well! Kindly arrange to send us the certificate of compliance (CoC) for the below Abcam products as desired by one of our customers : Cat No. Description ab97021 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) ab97049 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) ab97023 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) ab60314 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) ab97080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed ab65623 ECL Western Blotting Substrate Kit - 50 Tests ab52476 Rabbit monoclonal [EP1829Y] to CaMKII ab11800 Goat polyclonal to DCXR ab7260 Rabbit polyclonal to GFAP - Astrocyte Marker ab104224 Mouse monoclonal [1B7] to FOX3 ab76260 Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) ab64230 Mounting Medium ab82477 Rabbit polyclonal to Adenosine A1 Receptor ab3462 Rabbit polyclonal to alpha 1 Adrenergic Receptor

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2011

    Answer

    I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

    Read More

    Abcam Scientific Support

    Answered on Dec 21 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.