Mounting Medium With DAPI - Aqueous, Fluoroshield (ab104139)
Overview
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Product name
Mounting Medium With DAPI - Aqueous, Fluoroshield
See all Fluoroshield reagents -
Tested applications
Suitable for: IHC-Fr, ICC/IF, IHC-Pmore details -
General notes
Mounting Medium with DAPI ab104139 (previously called Fluoroshield Mounting Medium With DAPI) is an aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples. It is fortified with DAPI as a counter-stain for DNA (DAPI also stains RNA).
The formulation prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, R-Phycoerythrin (R-PE), Phyocyanin (PC), and Allophycocyanin (APC).
Fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, R-PE, PC and APC.
Mounting media productsFor fluorescent cell and tissue staining, Abcam recommends aqueous, anti-fade Fluorescence Mounting Medium ab104135, Mounting Medium with PI ab104129, and this product, Mounting Medium with DAPI ab104139. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI ab188804.
For chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend aqueous Mounting Medium ab64230, or organic Limonene Mounting Medium ab104141.
Procedure
- Bring the vial to room temperature.
- Rinse slide to be mounted with distilled or deionized water, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.
- Turn the vial upside down and open the dropper to remove any air bubbles.
- Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against paper towel.
- Let stand at room temperature for about 5 minutes.
- Apply cover slip carefully avoiding air bubbles.
- The specimen is ready for visualization under a microscope.
Seal the edges of cover slip with nail polish, any organic medium or Limonene mounting medium ab104141. If a coverslip is not sealed, air bubbles will appear in a few days.
Store the slide in the dark at 2-8°C.
Removal of Coverslip
Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deiononized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.Refractive Index
1.364 ± 0.002. This number applies to this mounting medium in solution. Refractive indexes change when the water solvent evaporates and mounting media dries on slides. We do not have the means to measure the refractive indexes of dry mounting mediums; however we expect the numbers to go higher when dried. The refractive index of water is 1.3330.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.0002% 4',6-Diamidino-2-phenylindole dihydrochloride, 84% Water -
Concentration information loading... -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
Images
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Immunohistochemistry (Frozen sections) - Fluoroshield Mounting Medium With DAPI (ab104139)This image is courtesy of an abreview submitted by Bryan Niedenberger.
Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS and stained with undiluted Fluoroshield Mounting Medium with DAPI (ab104139).
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Immunocytochemistry/ Immunofluorescence - Fluoroshield Mounting Medium With DAPI (ab104139)This image is courtesy of an Abreview submitted by Sebastian Alvarado.ab104139 worked exactly as it should in resin sections and stained heterochromatic areas of the nucleus.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (327)
ab104139 has been referenced in 327 publications.
- Eastlake K et al. Strain Specific Responses in a Microbead Rat Model of Experimental Glaucoma. Curr Eye Res 46:387-397 (2021). PubMed: 32842792
- Bou-Gharios J et al. The potential use of tideglusib as an adjuvant radio-therapeutic treatment for glioblastoma multiforme cancer stem-like cells. Pharmacol Rep 73:227-239 (2021). PubMed: 33140310
- Gao TY et al. Dynamic changes in the systemic immune responses of spinal cord injury model mice. Neural Regen Res 16:382-387 (2021). PubMed: 32859802
- Fu X et al. Osteopontin Regulates Endometrial Stromal Cell Migration in Endometriosis through the PI3K Pathway : Osteopontin Regulates Endometrial Cell Migration in Endometriosis. Reprod Sci 28:435-446 (2021). PubMed: 32909189
- DoGan E et al. The effects of PIKfyve inhibitor YM201636 on claudins and malignancy potential of nonsmall cell cancer cells. Turk J Biol 45:26-34 (2021). PubMed: 33597819