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Mounting Medium With DAPI - Aqueous, Fluoroshield (ab104139)

Reviews (8)Q&A (5)References (327)
Immunohistochemistry (Frozen sections) - Fluoroshield Mounting Medium With DAPI (ab104139)
  • Immunocytochemistry/ Immunofluorescence - Fluoroshield Mounting Medium With DAPI (ab104139)

Overview

  • Product name

    Mounting Medium With DAPI - Aqueous, Fluoroshield
    See all Fluoroshield reagents

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-P, In situ hybridizationmore details

  • General notes

    Mounting Medium with DAPI ab104139 (previously called Fluoroshield Mounting Medium With DAPI) is an aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples. It is fortified with DAPI as a counter-stain for DNA (DAPI also stains RNA).

    The formulation prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, R-Phycoerythrin (R-PE), Phyocyanin (PC), and Allophycocyanin (APC).

    Fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, R-PE, PC and APC.


    Mounting media products

    For fluorescent cell and tissue staining, Abcam recommends aqueous, anti-fade Fluorescence Mounting Medium ab104135, Mounting Medium with PI ab104129, and this product, Mounting Medium with DAPI ab104139. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI ab188804.

    For chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend aqueous Mounting Medium ab64230, or organic Limonene Mounting Medium ab104141. 

     

    Procedure

    - Bring the vial to room temperature.

    - Rinse slide to be mounted with distilled or deionized water, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.

    - Turn the vial upside down and open the dropper to remove any air bubbles.

    - Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against paper towel.

    - Let stand at room temperature for about 5 minutes.

    - Apply cover slip carefully avoiding air bubbles.

    - The specimen is ready for visualization under a microscope.

    Seal the edges of cover slip with nail polish, any organic medium or Limonene mounting medium ab104141. If a coverslip is not sealed, air bubbles will appear in a few days.

    Store the slide in the dark at 2-8°C.

    Removal of Coverslip
    Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deiononized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.

    Refractive Index
    1.364 ± 0.002. This number applies to this mounting medium in solution. Refractive indexes change when the water solvent evaporates and mounting media dries on slides. We do not have the means to measure the refractive indexes of dry mounting mediums; however we expect the numbers to go higher when dried. The refractive index of water is 1.3330.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab104139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
In situ hybridization
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
In situ hybridization
Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Frozen sections) - Fluoroshield Mounting Medium With DAPI (ab104139)
    Immunohistochemistry (Frozen sections) - Fluoroshield Mounting Medium With DAPI (ab104139)This image is courtesy of an abreview submitted by Bryan Niedenberger.

    Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS and stained with undiluted Fluoroshield Mounting Medium with DAPI (ab104139).

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Fluoroshield Mounting Medium With DAPI (ab104139)
    Immunocytochemistry/ Immunofluorescence - Fluoroshield Mounting Medium With DAPI (ab104139)This image is courtesy of an Abreview submitted by Sebastian Alvarado.

    ab104139 worked exactly as it should in resin sections and stained heterochromatic areas of the nucleus.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (327)

Publishing research using ab104139? Please let us know so that we can cite the reference in this datasheet.

ab104139 has been referenced in 327 publications.

  • Eastlake K  et al. Strain Specific Responses in a Microbead Rat Model of Experimental Glaucoma. Curr Eye Res 46:387-397 (2021). PubMed: 32842792
  • Bou-Gharios J  et al. The potential use of tideglusib as an adjuvant radio-therapeutic treatment for glioblastoma multiforme cancer stem-like cells. Pharmacol Rep 73:227-239 (2021). PubMed: 33140310
  • Gao TY  et al. Dynamic changes in the systemic immune responses of spinal cord injury model mice. Neural Regen Res 16:382-387 (2021). PubMed: 32859802
  • Fu X  et al. Osteopontin Regulates Endometrial Stromal Cell Migration in Endometriosis through the PI3K Pathway : Osteopontin Regulates Endometrial Cell Migration in Endometriosis. Reprod Sci 28:435-446 (2021). PubMed: 32909189
  • DoGan E  et al. The effects of PIKfyve inhibitor YM201636 on claudins and malignancy potential of nonsmall cell cancer cells. Turk J Biol 45:26-34 (2021). PubMed: 33597819
View all Publications for this product

Customer reviews and Q&As

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Submit a review Submit a question

1-10 of 13 Abreviews or Q&A

Excellent mounting media

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
Excellent mounting media, keeps stained slides for a long time. Make sure to apply and incubate it a bit before imaging, it takes a while for DAPI to get in every cell.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 07 2021

Glycogen synthase kinase-3 inhibition attenuates fibroblast activation and development of fibrosis following renal ischemia-reperfusion in mice.

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
tried many different product to get nuclear staining better and more clear with mounting solution but failed then I bought abcam ab104139 - Fluoroshield Mounting Medium With DAPI in 3T3L1 adipocyte cells as recommended in their datasheets and I never had any problem with it. I think its great product to use. I think this is best product to detect nuclear staining with mounting solution during in vivo and in vitro studies
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 17 2018

Best quality-price ratio

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
I've tried some others, but I'll definitively stick with this one. Nice stain and affordable price.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 31 2017

Excellent and convenient

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
I've used a number of various mounting media - SouthernBiotech, Invitrogen/Life Tech, Vectashield, and now this. This one is my favorite for several reasons: (1) Affordability, (2) Packaging, and (3) ease of use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 31 2017

Good Solution for fast Staining

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
It works as expected. Picture shows an example of DAPI positive nuclei.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 19 2016

Works great!

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
This dye works great for frozen or paraffin embedded skin tissue in all our experiments.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ahmar Aziz

Verified customer

Submitted May 05 2016

Excellent results

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS and stained with undiluted Fluoroshield Mounting Medium with DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Bryan Niedenberger

Verified customer

Submitted Mar 09 2016

Fantastic!

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
I was worried about some of the reviews but the price made it an easy decision. I was pretty happy since it worked exactly as it should in resin sections and stained heterochromatic areas of the nucleus
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sebastian Alvarado

Verified customer

Submitted Dec 08 2015

Question

Do you offer any discounts for teaching institutions?

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Answer

Thank you for your reply. While we do not generally give discounts for teaching institutions in particular, if you are starting a new lab, moving a lab, or a first-time grant recipient, you may qualify for our New Lab Discount Program:

https://www.abcam.com/newlab

I hope this helps, please let me know if you need any additional information or assistance.

Read More

Question

Do you sell DAPI or could you recommend a supplyer?

Read More
Answer

Thank you for contacting us.


We do not currently sell DAPI that is not contained in mounting media, but the red fluorescent DRAQ5 dye (ab108410) can be used in a very similar fashion to produce nuclear staining on fixed tissue. Alternatively, you can search biocompare.com for vendors that sell DAPI separately.


I hope this helps, please let me know if you need any additional information or assistance.

Read More

1-10 of 13 Abreviews or Q&A

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