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    products/reagents/native-lysis-buffer-ab156035.pdf

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Kits/ Lysates/ Other Tools and Reagents Lysis
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Native lysis Buffer (ab156035)

  • Datasheet
  • SDS
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SDS-PAGE - Native lysis Buffer (ab156035)

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    Overview

    • Product name

      Native lysis Buffer
      See all Lysis Buffer reagents
    • Tested applications

      Suitable for: WB, Sandwich ELISA, SDS-PAGE, IPmore details
    • General notes

      Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent extracts cytoplasmic, nuclear and membrane proteins. The detergent used in this buffer is relatively gentle and in many cases allows solubilization of protein complexes that retain enzymatic activity. On the flip side, the gentle nature of this lysis buffer means that it may result in extractions that are less complete than lysis buffers using more stringent detergents (e.g. ab156034). Native lysis buffer is compatible with many downstream applications, including enzyme activity assays, SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.

    Properties

    • Form

      Liquid
    • Storage instructions

      Store at +4°C.
    • Storage buffer

      pH: 7.50
      Constituents: 0.02% Beta glycerophosphate, 0.02% Sodium orthovanadate, 0.04% EGTA, 0.6% HEPES, 0.03% EDTA, 0.58% Sodium chloride, 1.5% Dodecyl maltoside, 0.11% Sodium pyrophosphate decahydrate
    • Concentration information loading...
    • Research areas

      • Kits/ Lysates/ Other
      • Tools and Reagents
      • Lysis

    Associated products

    • Related Buffer

      • 10% Lauryl Maltoside Solution (ab109857)
      • 15% Lauryl Maltoside Solution (ab109858)
    • Related Products

      • PMSF, protease inhibitor (ab141032)
      • Protease Inhibitor Cocktail (ab65621)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab156035 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent concentration.
    Sandwich ELISA
    Use at an assay dependent concentration.
    SDS-PAGE
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.
    Notes
    WB
    Use at an assay dependent concentration.
    Sandwich ELISA
    Use at an assay dependent concentration.
    SDS-PAGE
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.

    Images

    • SDS-PAGE - Native lysis Buffer (ab156035)
      SDS-PAGE - Native lysis Buffer (ab156035)
      HeLa cell extraction using ab156035.
      2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156035. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes: the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.
      BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.15mg of protein was recovered and 70% was in the soluble cleared cell lysate.

      Lane 1: MW marker
      Lane 2: Cleared lysate
      Lane 3: Non-soluble

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab156035? Please let us know so that we can cite the reference in this datasheet.

    ab156035 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    A good lysis buffer for both cell lysis prep and native gel running

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    I bought this buffer several times from different batches, it always can lysate cells efficiently. Besides, we test protein complex in cell lysates under native condition as well, results indicated that this buffer can keep protein complexes well and doesn't disrupt the complexity of protein scaffold.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 02 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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