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    products/reagents/normal-donkey-serum-ab7475.pdf

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Normal Donkey Serum (ab7475)

  • Datasheet
Submit a review Q&A (5)References (128)

Product price, shipping and contact information

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Functional Studies - Normal Donkey Serum (ab7475)
  • Functional Studies - Normal Donkey Serum (ab7475)

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Overview

  • Product name

    Normal Donkey Serum
    See all Donkey Serum reagents
  • Host species

    Donkey
  • Tested applications

    Suitable for: IHC (PFA fixed), Blocking, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

  • General notes

    Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.

    The donkey serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.

    Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised. 

    Serum can be used directly for blocking, or as a constituent of a blocking buffer.

     

     

    Strain: Mixed breed and sex.

    Raised in: Donkey

    Purity: Whole antiserum

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: Whole serum
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Serum Products

Associated products

  • Related Products

    • Normal Donkey serum (sterile) (ab138579)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
    • Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab97110)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed)
Use at an assay dependent dilution.
Blocking
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution.
Notes
IHC (PFA fixed)
Use at an assay dependent dilution.
Blocking
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution.

Images

  • Functional Studies - Normal Donkey Serum (ab7475)
    Functional Studies - Normal Donkey Serum (ab7475)Paul, Rajib et al., PloS one vol. 12,2 e0171285., Fig 5, doi:10.1371/journal.pone.0171285

    The sections were rinsed three times with 0.1 M Tris-buffered saline (TBS, 0.1 M; pH 7.4), incubated in 3% H2O2  in TBS, permeabilized with 0.3% Triton X-100, and blocked with 10% donkey serum containing 0.3% Triton X-100. The sections were incubated overnight with primary antibody for TH (1:700) in TBS, containing 2% donkey serum (ab7475) at 4°C and then incubated with HRP-conjugated secondary antibody (1:1000) in TBS for 1 h at room temperature.

  • Functional Studies - Normal Donkey Serum (ab7475)
    Functional Studies - Normal Donkey Serum (ab7475)Krzy?anowska, Weronika et al., PloS one?vol. 12,10 e0186243., Fig 6, doi:10.1371/journal.pone.0186243
    Anti-xCT rabbit (ab37185 Abcam) dilution: 1:200 Donkey anti-rabbit TR (ab6800 Abcam) dilution: 1:1000 Donkey normal serum (ab7475 Abcam) dilution: 10%

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (128)

Publishing research using ab7475? Please let us know so that we can cite the reference in this datasheet.

ab7475 has been referenced in 128 publications.

  • Alvarez KLF  et al. Protocol to assess the effects of dysfunctional human vascular smooth muscle cells on other brain cells using in vitro models of Alzheimer's disease. STAR Protoc 3:101149 (2022). PubMed: 35141568
  • Privat-Maldonado A  et al. Cold Atmospheric Plasma Does Not Affect Stellate Cells Phenotype in Pancreatic Cancer Tissue in Ovo. Int J Mol Sci 23:N/A (2022). PubMed: 35216069
  • Zywitza V  et al. Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction. Sci Rep 12:3100 (2022). PubMed: 35260583
  • Nemeth DP  et al. Dynamic Interleukin-1 Receptor Type 1 Signaling Mediates Microglia-Vasculature Interactions Following Repeated Systemic LPS. J Inflamm Res 15:1575-1590 (2022). PubMed: 35282272
  • Abdel-Razek O  et al. Role of Surfactant Protein D in Experimental Otitis Media. J Innate Immun 13:197-210 (2021). PubMed: 33556949
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Question

Could you be so kind and advise me the fluorescent marked secondary antibodies for ab109632 and ab421?

Thank you.

Best regards

Read More

Abcam community

Verified customer

Asked on Dec 05 2012

Answer

Thank you for contacting us.

Please try ab97099 and ab6717.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Dec 05 2012

Question

 I would like to inform you that I plan to use Peroxide/DAB the substrate and chromogen for horseradish peroxidase as detection system I appreciate you for your kind help                                                       with my best regards                                                    

Read More

Abcam community

Verified customer

Asked on Oct 11 2012

Answer

Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the choice will be personal preference of host. These products are below. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab16284: https://www.abcam.com/Rabbit-IgG-secondary-antibody-ab16284.html Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab97051: https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab97051.html Abcam also provides a number of IHC reagents and kits such as DAB substrate kits, donkey or goat sera and antigen retrieval buffers. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. I have provided links to a few of these products below. DAB substrate kit:https://www.abcam.com/DAB-substrate-kit-ab94665.html Donkey serum:https://www.abcam.com/Donkey-Serum-ab7475.html Goat serum:https://www.abcam.com/Goat-Serum-ab7481.html Antigen retrieval buffer:https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-9-0-ab93684.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Oct 11 2012

Question

I have one question for you. My customer wants to buy serum "goat" and "donkey" for blocking nonspecific effects in imunohistochemistry. Do you have something like this in your offer?

Thank you for your answer.

Best regards

Read More

Abcam community

Verified customer

Asked on Sep 19 2012

Answer

Thank you for contacting us.

The goat serum is ab7481; https://www.abcam.com/Goat-Serum-ab7481.html

Donkey serum ab7475; https://www.abcam.com/Donkey-Serum-ab7475.html

ab138478; https://www.abcam.com/Goat-Serum-sterile-ab138478.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Sep 19 2012

Question

J’aimerais savoir si vous avez les certificat d’analyse des produits suivants:
Rabbit serum ref: ab7487 lot 833644
Donkey serum ref: ab7475 lot: 854038
Sheep serum ref: ab7489 lot: GR66413-1
Merci d’avance pour votre réponse

Read More

Abcam community

Verified customer

Asked on Apr 16 2012

Answer



Veuillez trouver ci-joints les certificats d'analyse pour les produits :

Rabbit serum ref : ab7487 lot 833644
Donkey serum ref: ab7475 lot: 854038
Sheep serum ref: ab7489 lot: GR66413-1

Read More

Abcam Scientific Support

Answered on Apr 16 2012

Question

Should ab7475, donkey serum, and the other species sera be appropriate for FACS blocking as well?

Read More

Abcam community

Verified customer

Asked on Feb 21 2007

Answer

Thank you for your enquiry. ab7475 has not been tested in FACS, but the use of this product in flow cytometry/blocking step should be okay. This should be true for the other serum products - please check the datasheets. Please don't hesitate to contact us again if you have further questions.

Read More

Abcam Scientific Support

Answered on Feb 21 2007

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