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    products/reagents/normal-goat-serum-ab7481.pdf

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Normal Goat Serum (ab7481)

  • Datasheet
Reviews (2)Q&A (10)References (251)

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Functional Studies - Normal Goat Serum (ab7481)
  • Functional Studies - Normal Goat Serum (ab7481)

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Overview

  • Product name

    Normal Goat Serum
    See all Goat Serum reagents
  • Host species

    Goat
  • Tested applications

    Suitable for: Blocking, IHC-Fr, ICC/IF, IHC-Pmore details
  • General notes

    Normal goat serum ab7481 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.

    The goat serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.

    Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised. 

    Serum can be used directly for blocking, or as a constituent of a blocking buffer.

     

    Strain: Mixed breed and sex.

    Raised in: Goat

    Purity: Whole antiserum

    This product is for research use only and not intended for diagnostic or therapeutic use of any kind. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: Whole serum
  • Concentration information loading...
  • Reagent notes

    Strain: Mixed breed and sex.
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • IHC Tools/ Reagents
    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Serum Products

Associated products

  • Related Products

    • Normal Goat Serum (Sterile) (ab138478)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    • Goat Anti-Mouse IgG H&L (HRP) (ab6789)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent dilution.
Notes
Blocking
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent dilution.

Images

  • Functional Studies - Normal Goat Serum (ab7481)
    Functional Studies - Normal Goat Serum (ab7481)Dzinic, Sijana H et al., PloS one vol. 8,11 e74502., Fig 2, doi:10.1371/journal.pone.0074502

    Cells grown in 8-well chamber slides to 70% confluence were fixed with 4% paraformaldehyde (15 min at room temperature (RT)), and permeabilized with 100% ice cold methanol (10 min at 20°C). The slides were incubated with 10% normal goat serum (ab7481) in PBS for 1 hr, and incubated with anti-maspin (1:100) antibody alone or in a combination with either anti-lamin B (1:50), anti-HDAC1 (1:50) or anti-GRP78 (1:50) at 4°C overnight. Cells were washed and incubated for 2 hrs at room temperature (RT) with Alexa Fluor 488 (1:500) alone or in combination with Alexa Fluor 594 (1:500). The nuclei were counterstained with DAPI.
    DU145 cells infected with adenovirus expressing either maspinWT or maspinD346E Confocal immunofluorescence imaging of maspin (green) and nuclear envelope marker lamin B (red) in DU145 cells after infection.

  • Functional Studies - Normal Goat Serum (ab7481)
    Functional Studies - Normal Goat Serum (ab7481)Witasp, Anna et al., PloS one vol. 8,5 e63493., Fig 3, doi:10.1371/journal.pone.0063493
    Slides were pretreated with Hydrogen Peroxide Block followed by 15% normal goat serum (Abcam, Cambridge, UK) for 1 hour. The primary antibody, anti-PTX3, N-terminal antibody produced in rabbit was diluted 1:300 in PBS with 2.5% goat serum (ab7481), applied to slides and incubated for 3 hours at 4°C. The primary antibody was omitted in the negative controls. The PTX3 binding was revealed using a universal secondary antibody polymer formulation conjugated to horseradish peroxidase (HRP). The HRP activity was subsequently visualized with diaminobenzidine (DAB) substrate/chromogen and counterstaining with hematoxylin was performed.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (251)

Publishing research using ab7481? Please let us know so that we can cite the reference in this datasheet.

ab7481 has been referenced in 251 publications.

  • Reeves KM  et al. 18F-FMISO PET Imaging Identifies Hypoxia and Immunosuppressive Tumor Microenvironments and Guides Targeted Evofosfamide Therapy in Tumors Refractory to PD-1 and CTLA-4 Inhibition. Clin Cancer Res 28:327-337 (2022). PubMed: 34615724
  • Gao L  et al. Schisandrin A protects against isoproterenol-induced chronic heart failure via miR-155. Mol Med Rep 25:N/A (2022). PubMed: 34812475
  • Zyner KG  et al. G-quadruplex DNA structures in human stem cells and differentiation. Nat Commun 13:142 (2022). PubMed: 35013231
  • Hadanny A  et al. Effects of Hyperbaric Oxygen Therapy on Mitochondrial Respiration and Physical Performance in Middle-Aged Athletes: A Blinded, Randomized Controlled Trial. Sports Med Open 8:22 (2022). PubMed: 35133516
  • Vora SM  et al. Targeting stem-loop 1 of the SARS-CoV-2 5' UTR to suppress viral translation and Nsp1 evasion. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35149555
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 12 Abreviews or Q&A

Pax7 cells stained with ab187339 using normal goat serum ab7481

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
I've been using this normal goat serum for all of my blocking on mouse skeletal muscle frozen sections and I'm very happy with its performance.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 28 2021

Perfect for immunostain

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
I've been using this product for a couple of years as a blocking agent before an immunostain of cultured cells and tissue samples.
So far so good. I'll keep using this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Manuel Chacon

Verified customer

Submitted Oct 04 2021

Question

I bought normal goat serum and plan to use it as a blocking reagent before an immunostain of cells and tissue. Should I heat-inactivate the serum before use?

Read More

Abcam community

Verified customer

Asked on Jan 28 2016

Answer

There is no need to heat-inactivate serum if you are using it as a blocking reagent before staining fixed cells or tissue. Heat-inactivation is only necessary if you are using the serum for tissue culture. (The heat inactivates complement, and this eliminates complement-mediated cell lysis).

Read More

Tom Ruyle

Abcam Scientific Support

Answered on Jan 28 2016

Question

I'm trying to understand the difference between ab138478and ab7481. I will be using the serum for blocking of IHC on frozen tissue with fluorescent tagged antibodies.

Read More

Abcam community

Verified customer

Asked on Dec 07 2013

Answer

ab138478 is sterilized, whereas ab7481 is not. That is the only difference between the two products and the reason that ab138478 is more expensive.

Read More

Kevin Hanson

Abcam Scientific Support

Answered on Dec 07 2013

Question

Kindly advise if ab7481 is guaranteed by Abpromise as a blocking for IP procedure.

Read More

Abcam community

Verified customer

Asked on Mar 12 2012

Answer

ab7481 was not experimentally tested as blocking agent in IP procedures. But I am confident that it should be suitable in pre-clearing steps etc in the IP procedure. Please follow this link to a protocol for IP:
https://www.abcam.com/index.html?pageconfig=resource&rid=11385

Read More

Abcam Scientific Support

Answered on Mar 12 2012

Question

On the datasheet of these sera I have a concentration of 86mg/ml for the goat serum (8,6% concentration) and 71mg/ml for the rabbit serum (7,1% concentration). So no chance I can use them at 10% for IHC blocking on free-floating sections? 

Read More

Abcam community

Verified customer

Asked on Feb 07 2012

Answer

Those values are for whole protein value which we supply as a customer service. The total protein value is not considered when making blocking solutions as the percentage used is of the whole serum. Therefore to create a 100 ml or a 10% IHC block you would use 10 ml of serum in 90 ml of diluent.

Read More

Abcam Scientific Support

Answered on Feb 07 2012

Question

What is the optimal dilution to use these sera for blocking in IHC on free-floating sections?

Read More

Abcam community

Verified customer

Asked on Feb 01 2012

Answer

According to the standard protocol we have for free floating sections, we recommend to incubate the sections with 0.1M PB/0.3% triton/1% blocking serum for at least 1 h.
The whole protocol can be found here:

https://www.abcam.com/index.html?pageconfig=resource&rid=13633

As always certain steps including the amount of blocking agent might have to be optimized according to circumstances like sample, results, etc.

Read More

Abcam Scientific Support

Answered on Feb 01 2012

Question

I would like to know if I need to heat-inactivate goat serum before aliquoting and storing, and the best protocol to do it.

Read More

Abcam community

Verified customer

Asked on Feb 01 2012

Answer

To use this serum for IHC in the blocking step, it does not have to be heat treated. The heat treatment is done to inactivate complement in FCS meant for cell culture for example.

I recommend to aliquot and freeze the serum for storage.

Read More

Abcam Scientific Support

Answered on Feb 01 2012

Question

What is the optimal dilution for ab7481?

Read More

Abcam community

Verified customer

Asked on Jan 31 2012

Answer

The dilution that works best for your experiment depends on multiple variables so you should do some titrations to find the best dilution. A good starting point for ab7481 is 10%.

Read More

Abcam Scientific Support

Answered on Jan 31 2012

Question

Was this product heat inactivated?

Read More

Abcam community

Verified customer

Asked on Jun 07 2007

Answer

ab7481 (Normal Goat Serum) is not heat-inactivated.

Read More

Abcam Scientific Support

Answered on Jun 07 2007

1-10 of 12 Abreviews or Q&A

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