For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/reagents/nuclear-yellow-hoechst-s769121-ab138903.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Tools and Reagents Fluorescent dyes and reagents
Share by email

Nuclear yellow (Hoechst S769121) (ab138903)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Nuclear yellow (Hoechst S769121) (ab138903)
  • Nuclear yellow (Hoechst S769121) (ab138903)

You may also be interested in

Assay
Product image
Propidium Iodide (ab14083)
Biochemical
Product image
Bisbenzimide H 33258 (Hoechst 33258), adenine-thymine selective DNA fluorescent stain (ab145596)
Dye
Product image
DAPI Staining Solution (ab228549)

View more associated products

Overview

  • Product name

    Nuclear yellow (Hoechst S769121)
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • General notes

    The Hoechst stains are a family of fluorescent stains for labeling DNA used in fluorescence microscopy and flow cytometry. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria.

    The long-wavelength tracer Nuclear yellow is often combined with the popular retrograde tracer True Blue for two-color neuronal mapping. In neuronal cells, Nuclear yellow primarily stains the nucleus with yellow fluorescence, whereas True Blue is a UV light–excitable, divalent cationic dye that stains the cytoplasm with blue fluorescence. Both Nuclear yellow and True Blue are stable when subjected to immunohistochemical processing and can be used to photoconvert DAB into an insoluble, electron-dense reaction product.

    Molecular Weight: 651.01
    Appearance: Light yellow powder
    Solvents: water or dimethylsulfoxide (DMSO)

    SPECTRAL PROPERTIES:

    Excitation: Exmax 355 nm

    Emission: Emmax 495 nm*

Properties

  • Form

    Lyophilized
  • Storage instructions

    Store at -20°C. Store In the Dark.
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Fluorescent dyes and reagents
    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • Nuclear

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab138903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Images

  • Immunocytochemistry/ Immunofluorescence - Nuclear yellow (Hoechst S769121) (ab138903)
    Immunocytochemistry/ Immunofluorescence - Nuclear yellow (Hoechst S769121) (ab138903)

    ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150064 Alexa Fluor® 594 donkey anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Nuclear Yellow (Hoechst S769121) (ab138903) was used to stain the cell nuclei (purple/blue) at a 250µg/ml dilution with a 60 minute incubation.

  • Nuclear yellow (Hoechst S769121) (ab138903)
    Nuclear yellow (Hoechst S769121) (ab138903)
    Chemical structure of ab138903 Nuclear yellow (Hoechst S769121).

    Chemical structure of ab138903 Nuclear yellow (Hoechst S769121).

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab138903? Please let us know so that we can cite the reference in this datasheet.

ab138903 has been referenced in 2 publications.

  • Bray JK  et al. Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury. Cell Death Dis 11:138 (2020). PubMed: 32080178
  • Hillje AL  et al. TRIM32-dependent transcription in adult neural progenitor cells regulates neuronal differentiation. Cell Death Dis 4:e976 (2013). PubMed: 24357807

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab138903.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.