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PAP pen (ab2601)

Reviews (4)Q&A (5)References (19)
PAP pen (ab2601)

    Overview

    • Product name

      PAP pen

    • General notes

      The PAP pen is a special marking pen that provides a thin film-like green-tinged hydrophobic barrier when a circle is drawn gently around a specimen on a slide. Gentle application of the pen tip while drawing is advised.

      This water repellent barrier keeps staining reagents localized on the tissue sections, prevents mixing of reagents when differentially staining two sections on the same slide, and allows use of less reagents per section.

      Insoluble in alcohol and acetone, PAP Pen markings can be optionally removed with xylene after the staining procedure is complete.

      PAP Pen is good for approximately 500 uses.

      The formulation of PAP pen has been optimised to increase water repellence and tolerance against alcohol and surface active agent, and is now a lighter colour.

    Properties

    Images

    • PAP pen (ab2601)
      PAP pen (ab2601)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (19)

    Publishing research using ab2601? Please let us know so that we can cite the reference in this datasheet.

    ab2601 has been referenced in 19 publications.

    • Banovic P  et al. Shared Odds of Borrelia and Rabies Virus Exposure in Serbia. Pathogens 10:N/A (2021). PubMed: 33800537
    • Opzoomer JW  et al. ImmunoCluster provides a computational framework for the nonspecialist to profile high-dimensional cytometry data. Elife 10:N/A (2021). PubMed: 33929322
    • Wörsdörfer P  et al. Generation of Vascularized Neural Organoids by Co-culturing with Mesodermal Progenitor Cells. STAR Protoc 1:100041 (2020). PubMed: 33111089
    • Jandrey EHF  et al. Quantification of Tumor Cell Adhesion in Lymph Node Cryosections. J Vis Exp N/A:N/A (2020). PubMed: 32090991
    • Lugos MD  et al. Using Immunohistochemistry Without Linkers to Determine the Optimum Concentrations of Primary Antibodies for Immunofluorescence Staining of Formalin-fixed Paraffin-embedded Tissue Sections. Appl Immunohistochem Mol Morphol 28:249-257 (2020). PubMed: 32197004
    View all Publications for this product

    Customer reviews and Q&As

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    1-9 of 9 Abreviews or Q&A

    super pen

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    The pen works well. It`s easy to use and dries quickly. The barrier is precise and reliable - even after 24h incubation. It is resistant to buffer but can be removed with xylene.The pen is a great help in the IHC when there are multiple tissue samples on the same slide. And you save a lot of reagent, because you can frame the samples as small as possible.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 06 2018

    high quality PAP pen

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    abreview image
    This PAP pen is very easy to use. It dries quickly. It is strong enough to create and maintain a boundary between tissue sections. I usually stain secondary antibody control on the same slide with primary antibody, so I divided the section with the PAP pen. After overnight incubation with antibody, the boundary still functional and there was no mixing between adjacent drop of solutions. Picture was taken after overnight incubation.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 02 2016

    PAP pen

     Good Good 4/5 (Ease of Use)
    Abreviews
    abreview image
    PAP pen dries quickly and forms a hydrophobic layer that is not washed off by 0.1% Triton X-100 in PBS. The only concern I have is that the barrier is often difficult to dry after washing, so extra care must be taken to avoid mixing reagents between adjacent sections.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Bryan Niedenberger

    Verified customer

    Submitted Aug 31 2016

    Barrier pen

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Precise and reliable barrier pen. After application it dries rather quick and after that there's no leakage. Found it very useful for IHC when I had to isolate from each other few tissue samples on the same slide. It resists incubation in buffers with Tween-20. Soluble in Xylene.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Vasily Gnyubkin

    Verified customer

    Submitted May 02 2014

    Question

    Can the PAP pen be used at temperatures around 200°C?

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    Answer

    The PAP pen can be used at temperatures as high as 48°C (120°F). When working with temperatures higher than 48°C, the protective barrier may be compromised and loses its water-repellant properties.

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    Question

    I have a customer who is interested in AB2601 PAP PEN, it is indicated that each unit is good for approximately 500 uses. The customer wants to know what is the volume in the per unit ? I am looking forward to your reply. .

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    Answer

    Thank you for your enquiry. We have not calculated the volume of liquid used per unit, however I can confirm that one unit is equivalent to drawing with the pen along the four sides of a standard IHC slide, such that a hydrophobic barrier is created to ensure the tissue remains moist upon application of antibody. I hope this information will help your customer.

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    Question

    Is the PAP pen incompatible with solutions containing 50% formamide? I used a PAP pen to draw a barrier around tissue sections on glass slides, then overlaid the sections with a hybridization solution containing 50% formamide and 50% standard saline citrate (aqueous NaCl and sodium citrate). After an overnight incubation at 42 degrees in a humid chamber, some of the PAP barriers appeared to have partially disappeared--dissolved? The hybridization solution had flowed over, and there were floating green flecks (like bits of the barrier). I redrew the barrier before doing additional incubations in aqueous solutions, but the redrawn barriers seemed to have trouble adhering to the slides. I've used PAP pens before with aqueous solutions, with good results. So I wonder if the problem is the formamide.

    Read More
    Answer

    Thank you for your enquiry. The PAP pen has been used for IHC only. You should wipe the section in a "square- coverslip" fashion if you wish to use it for ISH. (At least the size of the cover slip.) The line that is drawn must be very dry before placing a small amount of probe on the tissue. I am unsure whether formamide is a xylene solvent, however we do not believe that the PAP pen will withstand formamide;if you use the pen on the outside of the cover slip that may work. Alternatively you could try using barrier slides? Good luck with your research.

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    Question

    Can the tissue section be encircled with the PAP-pen when the surface is wet?

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    Answer

    No, we recommend wiping the tissue dry before applying the pen to ensure the barrier does not spread across the tissue.

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    Question

    What is the thickness of the pen.

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    Answer

    The tip of the Pap pen is about 3 mm thick and tapers off to about 1 mm.

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