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Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

  • Datasheet
  • SDS
Reviews (3)Q&A (2)References (16)

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SDS-PAGE - Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

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    Overview

    • Product name

      Prestained Protein Ladder – Broad molecular weight (10-245 kDa)
      See all Prestained Protein Ladder reagents
    • Tested applications

      Suitable for: SDS-PAGE, WBmore details
    • General notes

      Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).

      This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.

      Key product features:

      • Broad range: 10-245 kDa
      • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
      • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
      • Sharp bands
      • Recommended loading: ~1.5 - 2.5µl

       

      Review other protein ladders in the unstained and prestained protein ladder guide.

      This product was previously called Prism Ultra Protein Ladder (10-245 kDa).

      Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

    Properties

    • Form

      Liquid
    • Storage instructions

      Please see notes section.
    • Storage buffer

      pH: 7.5
      Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol
    • Concentration information loading...
    • Research areas

      • Kits/ Lysates/ Other
      • Tools and Reagents
      • Western Blot Tools and Reagents
      • Protein ladders
    • Alternative names

      • Molecular weight marker
      • Prism protein ladder
      • SDS PAGE protein ladder prestained
      • WB prestained protein ladder
      • Western blot prestained protein ladder
      see all

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab116028 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    SDS-PAGE
    Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
    WB
    Use at an assay dependent concentration.

    ~1.5-2.5 µl per well for general western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

    Notes
    SDS-PAGE
    Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
    WB
    Use at an assay dependent concentration.

    ~1.5-2.5 µl per well for general western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

    Images

    • SDS-PAGE - Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
      SDS-PAGE - Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
      Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MOPS buffer, Gel 3: Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (16)

    Publishing research using ab116028? Please let us know so that we can cite the reference in this datasheet.

    ab116028 has been referenced in 16 publications.

    • Thejer BM  et al. PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth. BMC Mol Cell Biol 21:24 (2020). PubMed: 32245408
    • Depoix CL  et al. Effects of chemotherapy on placental development and function using in vitro culture of human primary cytotrophoblasts. Invest New Drugs 38:547-557 (2020). PubMed: 31155684
    • Negri S  et al. Group 1 metabotropic glutamate receptors trigger glutamate-induced intracellular Ca2+ signals and nitric oxide release in human brain microvascular endothelial cells. Cell Mol Life Sci 77:2235-2253 (2020). PubMed: 31473770
    • Chowdhury P  et al. Non-synonymous amino acid alterations in PfEBA-175 modulate the merozoite ligand's ability to interact with host's Glycophorin A receptor. Infect Genet Evol 85:104418 (2020). PubMed: 32561295
    • Ghoshal S  et al. Population genetic and biophysical evidences reveal that purifying selection shapes the genetic landscape of Plasmodium falciparum RH ligands in Chhattisgarh and West Bengal, India. Malar J 19:367 (2020). PubMed: 33054833
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Novel Insights Into the Role of UBE3A in Regulating Apoptosis and Proliferation

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    user friendly and stable after transfer
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Yonatan Feuermann

    Verified customer

    Submitted Jun 04 2020

    Prestained ladder works at LICOR

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    The ladder works perfectly at 2.5 ul (down to 1 ul) and also shows nice crisps bands not only on the nitrocellulose membrane but also when analysed at the LICOR. Same if not even a touch better results then comparable ladders from more expensive competitors.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Sebastian Bohm

    Verified customer

    Submitted Jun 22 2018

    One of the best Pre stained protein ladder

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    I have used 1.8 ul per well and get a clear band on transfer to membrane.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Vicky Valued Customer

    Verified customer

    Submitted Oct 10 2016

    Question

    Do you have any apparent sizing for 4-12% Bis-Tris? I've used it on a NuPAGE Novex 4-12% Bis-Tris Protein Gel with MOPS running buffer.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 24 2015

    Answer

    The apparent sizes will be the same as the ones in the second gel on the online datasheet, which is a 10% Bis-Tris gel, MOPS running buffer. The distribution will be different, with a little more separation of the higher molecular weight proteins at the 4% end of your gel compared to the 10% gel on the datasheet.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Jul 24 2015

    Question

    Are these histone tagged proteins?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2012

    Answer

    I can confirm that ab116027, ab116028, and ab116029 do not contain histone tagged proteins.

    Read More

    Abcam Scientific Support

    Answered on Sep 07 2012

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