Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa) (ab116029)
Overview
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Product name
Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa)
See all Prestained Protein Ladder reagents -
Tested applications
Suitable for: WB, SDS-PAGEmore details -
General notes
Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
This product is also sold by Expedeon, an Abcam company as RunBlue™ TriColor Prestained Protein Ladder: NXA6050. This product was previously called Prism Ultra Protein Ladder (3.5 - 245 kDa).
This prestained protein ladder is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Key product features:
- Broad range: 3.5-245 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
- Sharp bands
- Recommended loading: ~1.5 - 2.5µl
Review other protein ladders in the unstained and prestained protein ladder guide.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks). Store at -20°C. -
Storage buffer
pH: 7.5
Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol -
Concentration information loading... -
Research areas
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Alternative names
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
see all
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab116029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.
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| SDS-PAGE |
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis
on 15-well or 10-well mini-gel, respectively.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel. |
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SDS-PAGE
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. |
Images
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SDS-PAGE - Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa) (ab116029)
Gel 1: Tris-Glycine (4-15%) SDS buffer; Gel 2: Bis-Tris (4-12%) MOPS buffer; Gel 3: Bis-Tris (4-12%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
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SDS-PAGE - Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa) (ab116029)Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab116029 has been referenced in 3 publications.
- Idrees M et al. SHP2 Nuclear/Cytoplasmic Trafficking in Granulosa Cells Is Essential for Oocyte Meiotic Resumption and Maturation. Front Cell Dev Biol 8:611503 (2020). PubMed: 33553147
- Idrees M et al. PTPN11 (SHP2) Is Indispensable for Growth Factors and Cytokine Signal Transduction During Bovine Oocyte Maturation and Blastocyst Development. Cells 8:N/A (2019). PubMed: 31635340
- Idrees M et al. The PPARd Agonist GW501516 Improves Lipolytic/Lipogenic Balance through CPT1 and PEPCK during the Development of Pre-Implantation Bovine Embryos. Int J Mol Sci 20:N/A (2019). PubMed: 31810173