Prestained Protein Ladder – Extra broad molecular weight (6.5 – 270 kDa) (ab234592)
Overview
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Product name
Prestained Protein Ladder – Extra broad molecular weight (6.5 – 270 kDa)
See all Prestained Protein Ladder reagents -
Tested applications
Suitable for: WB, SDS-PAGEmore details -
General notes
Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa. This prestained protein ladder allows you to monitor protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key product features:
- Broad range: 6.5-270 kDa
- Ready to use: The ladder is supplied in the gel loading buffer and is ready to use.
- Easily identified: Proteins are covalently coupled with a blue chromophore except three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa).
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Review other protein ladders in the unstained and prestained protein ladder guide.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.50
Preservative: 0.01% Sodium azide
Constituents: 1.37% Tris phosphate, 0.03% EDTA, 2% Sodium lauryl sulfate, 0.15% DTT, 33% Glycerol -
Concentration information loading... -
Research areas
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Alternative names
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 μl. |
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| SDS-PAGE |
Use at an assay dependent concentration.
For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively. |
| Notes |
|---|
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WB
Use at an assay dependent concentration. For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 μl. |
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SDS-PAGE
Use at an assay dependent concentration. For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively. |
Images
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SDS-PAGE - Prestained Protein Ladder – Extra broad molecular weight (6.5 – 270 kDa) (ab234592)
SDS-PAGE analysis with ab234592.
Gel 1: Tris-Glycine 4-20%
Gel 2: Bis-Tris 4-12% MES buffer.
The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab234592 has been referenced in 1 publication.
- Bhattacharya A et al. Oncogenes overexpressed in metastatic oral cancers from patients with pain: potential pain mediators released in exosomes. Sci Rep 10:14724 (2020). PubMed: 32895418