Prestained Protein Ladder – Mid-range molecular weight (10 – 180 kDa) (ab234617)
Overview
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Product name
Prestained Protein Ladder – Mid-range molecular weight (10 – 180 kDa)
See all Prestained Protein Ladder reagents -
Tested applications
Suitable for: WB, SDS-PAGEmore details -
General notes
Prestained Protein Ladder ab234617 is a blue protein standard with 11 pre-stained proteins covering a range of MW from 10-180 kDa. ab234617 is designed for monitoring protein seperation in SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key product features:
- Broad range: 10-180 kDa
- Ready to use: The protein ladder is supplied in the gel loading buffer and is ready to use.
- Easily identified: Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer).
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μl per well for general Western transferring.
Review other protein ladders in the unstained and prestained protein ladder guide.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.50
Constituents: 0.44% Tris phosphate, 2% Sodium lauryl sulfate, 0.003% DTT, 21.6% Urea, 15% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Alternative names
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 1.5-2.5 μl. |
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| SDS-PAGE |
Use at an assay dependent concentration.
For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 1.5-2.5 μl. |
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SDS-PAGE
Use at an assay dependent concentration. For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively. |
Images
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SDS-PAGE - Prestained Protein Ladder – Mid-range molecular weight (10 – 180 kDa) (ab234617)
SDS-PAGE analysis with ab234617.
Gel 1: Tris-Glycine 10%
Gel 2: Bis-Tris 4-12% MOPS buffer
Gel 3: Bis-Tris 4-12% MES buffer.
The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab234617 has not yet been referenced specifically in any publications.