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  1. Link

    products/reagents/protein-block-ab156024.pdf

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Kits/ Lysates/ Other Kits IHC tools Block Protein Block
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Protein Block (ab156024)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (5)

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Overview

  • Product name

    Protein Block
  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-P, ELISAmore details
  • General notes

    Protein Block has been developed to use with immunolabeling techniques for the reduction of nonspecific background staining while simultaneously reducing the handling of animal serums in the laboratory. The need to match species with the secondary antibody is eliminated due to the lack of normal serum in this product. Protein Block has been shown to be effective for immunohistochemical, ELISA, blot and In-situ techniques and requires no mixing or diluting.

    Reagent: 60 ml

    Procedure:

    Immunohistochemistry:

    Incubate tissue section for 5 minutes at either room temperature or 37°C prior to application of the primary antibody. After incubation, rinse once in buffer (Optional) (NOTE: Do not incubate tissue sections in excess of 10 minutes or a reduction in desired staining may occur).

    For bulk staining, pour Protein Block in a covered staining tray and dip slides for 5 minutes. Replace with fresh Protein Block after 5-10 uses. This step can be performed at the time of deparaffinization.

    For antibodies with particularly high background staining, dilute Protein Block in PBS (1/5 - 1/10) and use as a wash buffer in addition to the blocking step.

    ELISA:

    Add Protein Block to microtiter well and incubate for 2-10 minutes prior to addition of sample. Rinse, and continue procedure. (NOTE: Do not incubate in excess of 10 minutes).

    Chemiluminescent Blot:

    Protein Block has been reported to be an effective blocker for this technique with incubation times of one hour at room temperature or overnight at 4°C.

    IHC Protocol Summary

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C.
  • Storage buffer

    Constituents: 0.002% Potassium chloride, 1% BSA, 1% Sodium chloride, 1% Gelatin, 1% Triton-X-100, 0.014% Dibasic monohydrogen sodium phosphate, 1% Monobasic dihydrogen potassium phosphate
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Block
    • Protein Block

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab156024? Please let us know so that we can cite the reference in this datasheet.

ab156024 has been referenced in 5 publications.

  • Wells AI  et al. Human FcRn expression and Type I Interferon signaling control Echovirus 11 pathogenesis in mice. PLoS Pathog 17:e1009252 (2021). PubMed: 33513208
  • Harvestine JN  et al. Osteogenic preconditioning in perfusion bioreactors improves vascularization and bone formation by human bone marrow aspirates. Sci Adv 6:eaay2387 (2020). PubMed: 32095526
  • Knier NN  et al. Comparing the fate of brain metastatic breast cancer cells in different immune compromised mice with cellular magnetic resonance imaging. Clin Exp Metastasis 37:465-475 (2020). PubMed: 32533389
  • Devarasetty M  et al. Simulating the human colorectal cancer microenvironment in 3D tumor-stroma co-cultures in vitro and in vivo. Sci Rep 10:9832 (2020). PubMed: 32555362
  • Harvestine JN  et al. Cell-secreted extracellular matrix influences cellular composition sequestered from unprocessed bone marrow aspirate for osteogenic grafts. Biomater Sci 7:2091-2101 (2019). PubMed: 30869662

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Amazing product..easy to handle.

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
IHC staining is always time taking process. Although many reagents need to prepared from the beginning of the process. Abcam premade protein block is very helpful in the process and it works amazing. The handling of the solution very easy just need to drop the solution on the tissue. we always use many Abcam products. I have used this product for staining NHERF-1 protein in human fetal membrane tissues and it works simply superb. Thank you, Abcam for this product. I
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 01 2021

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