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    products/reagents/protein-g-resin-amintra-ab270309.pdf

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Protein G Resin - Amintra (ab270309)

  • Datasheet
  • SDS
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Overview

  • Product name

    Protein G Resin - Amintra
  • General notes

    Protein G Resin - Amintra (ab270309) is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to highly cross-linked 4% agarose. The binding capacity of Protein G Resin - Amintra is greater than 30mg human lgG/mL medium. With the high binding capacity and chemical stability, very high batch-to-batch reproducibility, which ensures excellent performance on isolation of immune complexes.

    This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Protein G Resin. Expedeon product codes APG0005 (5 mL Medium), APG0025 (25 mL Medium) and APG0100 (100 mL Medium) are the same as the current sizes: 5 mL, 25 mL, and 100 mL, respectively.

    Characterization of Resin:

    • Supporting matrix: Highly crosslinked 4% agarose supplied as a 50% slurry
    • Ligand: Recombinant Protein G
    • Bead size range: 45-165 µm
    • pH stability: long term: pH 3 – 9 –short term: pH 2 – 10
    • Typical binding capacity: >30 mg Human IgG /ml resin
    • Maximum pressure: 0.3MPa (3 bar)
    • Chemical stability: High. The IgG binding capacity and recovery was maintained after storage for:
        1. 7 days at 37° in:
          • 1 M acetic acid pH 2.0,  20 mM sodium phosphate, 1% SDS, pH 7.0
          • 6 M Guandine-HCl, pH 7.0
          • 70% ethanol
        2. 2 hours at room temperature in:
          • 0.1 M HCl, pH 1.0
          • 8 M urea, pH 10.5
          • 0.1 M Glycine-NaOH, pH 11.0
    • Solubility in water: Insoluble

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at Room Temperature. Store at Room Temperature. Store at +4°C. Do Not Freeze. Please see notes section.
  • Storage buffer

    pH: 6
    Constituent: 20% Ethanol
  • Concentration information loading...

Protocols

  • Protocol Booklet

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Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab270309? Please let us know so that we can cite the reference in this datasheet.

ab270309 has been referenced in 1 publication.

  • Muñoz IM  et al. Phosphoproteomic screening identifies physiological substrates of the CDKL5 kinase. EMBO J 37:N/A (2018). PubMed: 30266825

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