Protein G Resin - Amintra (ab270309)

Protein G Resin - Amintra (ab270309) is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to highly cross-linked 4% agarose. The binding capacity of Protein G Resin - Amintra is greater than 30mg human lgG/mL medium. With the high binding capacity and chemical stability, very high batch-to-batch reproducibility, which ensures excellent performance on isolation of immune complexes.

This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Protein G Resin. Expedeon product codes APG0005 (5 mL Medium), APG0025 (25 mL Medium) and APG0100 (100 mL Medium) are the same as the current sizes: 5 mL, 25 mL, and 100 mL, respectively.

Characterization of Resin:

• Supporting matrix: Highly crosslinked 4% agarose supplied as a 50% slurry
• Ligand: Recombinant Protein G
• Bead size range: 45-165 µm
• pH stability: long term: pH 3 – 9 –short term: pH 2 – 10
• Typical binding capacity: >30 mg Human IgG /ml resin
• Maximum pressure: 0.3MPa (3 bar)
• Chemical stability: High. The IgG binding capacity and recovery was maintained after storage for:

1. 1. 1. 7 days at 37° in:
         • 1 M acetic acid pH 2.0,  20 mM sodium phosphate, 1% SDS, pH 7.0
         • 6 M Guandine-HCl, pH 7.0
         • 70% ethanol
      2. 2 hours at room temperature in:
         • 0.1 M HCl, pH 1.0
         • 8 M urea, pH 10.5
         • 0.1 M Glycine-NaOH, pH 11.0

• Solubility in water: Insoluble