Unstained Protein Ladder (10 - 200 kDa) (ab234618)
Overview
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Product name
Unstained Protein Ladder (10 - 200 kDa) -
Tested applications
Suitable for: WB, SDS-PAGEmore details -
General notes
Unstained Protein Ladder ab234618 contains 12 unstained recombinant proteins covering a wide range of molecular weights from 10-200 kDa.
This unstained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key features include:
- Broad range: 10-200 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easily identified: The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25 kDa and 85 kDa protein bands serving as the two reference bands.
5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel and for Western transferring.
Learn more about our unstained and prestained protein ladders in our guide.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.50
Constituents: 0.44% Tris phosphate, 2% Sodium lauryl sulfate, 0.003% DTT, 21.6% Urea, 15% Glycerol -
Concentration information loading...
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 5 µl. |
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SDS-PAGE |
Use at an assay dependent concentration.
For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 5 µl per loading for 15-well or 10-well mini-gel. |
Notes |
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WB
Use at an assay dependent concentration. For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 5 µl. |
SDS-PAGE
Use at an assay dependent concentration. For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 5 µl per loading for 15-well or 10-well mini-gel. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab234618 has not yet been referenced specifically in any publications.