Unstained Protein Ladder (10 - 200 kDa) (ab234618)
Overview
-
Product name
Unstained Protein Ladder (10 - 200 kDa) -
Tested applications
Suitable for: WB, SDS-PAGEmore details -
General notes
Unstained Protein Ladder ab234618 contains 12 unstained recombinant proteins covering a wide range of molecular weights from 10-200 kDa.
This unstained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key features include:
- Broad range: 10-200 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easily identified: The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25 kDa and 85 kDa protein bands serving as the two reference bands.
5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel and for Western transferring.
Learn more about our unstained and prestained protein ladders in our guide.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.50
Constituents: 0.44% Tris phosphate, 2% Sodium lauryl sulfate, 0.003% DTT, 21.6% Urea, 15% Glycerol -
Concentration information loading...
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 5 µl. |
|
| SDS-PAGE |
Use at an assay dependent concentration.
For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 5 µl per loading for 15-well or 10-well mini-gel. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 5 µl. |
|
SDS-PAGE
Use at an assay dependent concentration. For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 5 µl per loading for 15-well or 10-well mini-gel. |
Images
-
Unstained Protein Ladder (10 - 200 kDa) (ab234618)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab234618 has not yet been referenced specifically in any publications.