Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (ab267479)
Key features and details
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Plasma, Serum
Overview
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Product name
Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81)
See all Exosome kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Exosome 3 = 10% Inter-assay Sample n Mean SD CV% Exosomes 4 = 11% -
Sample type
Serum, Plasma -
Species reactivity
Reacts with: Human -
Product overview
Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
The kit is intended for the immunoisolation (immunomagnetic or FACS) and Flow Cytometry analysis of pre-enriched CD9+ human exosomes from plasma.
Visit our FAQs page for tips and troubleshooting.
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Notes
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.
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Platform
Flow cytometer
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 25 tests Anti-CD81 PE (Clone M38) 1 x 125µl Assay Buffer 10X 1 x 10ml CD9+ Capture Beads 1 x 1250µl -
Relevance
Exosomes are best defined as extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. This liberates intraluminal vesicles (ILVs) into the extracellular milieu and the vesicles thereby released are what we know as exosomes. They contain abundant proteins, DNA, mRNA, and non-coding RNAs. They are now considered as an additional mechanism for intercellular communication, allowing cells to exchange proteins, lipids and genetic material. Increasing studies have shown that exosomes play an important role in tumour initiation, growth, progression, metastasis, drug resistance and immune escape.
Images
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Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479)
FSC vs SSC (A) and FL3 vs FL4 (B).
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Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479)Cell culture exosomes, pre-enriched using Total Exosome Isolation from PC3 Cell Culture Media (A) and human plasma (B), were resuspended in PBS and bound to CD9-capture beads during an overnight incubation. The following day the bead-bound exosomes were direct stained with primary antibody detection (CD9/CD81-PE) and analyzed by flow cytometry.
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Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479)Dynamic range of the assay analyzed by flow cytometry. Relationship between background noise and specific signal at different exosome concentrations.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab267479 has not yet been referenced specifically in any publications.